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Cell No. : Cell Name
RCB0069 : RLC-16  update : 2022/10/06
CommentEpithelial cell from rat liver
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takaoka, Toshiko
Originator Takaoka, Toshiko
Year of deposit 1986
Animal rat < Mammals
Genus Rattus
Species norvegicus
Gender Female
Tissue liver
Classification other
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_3861
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Chromosome mode 33-41(50) : /36(5),37(3),38(9),39(10),40(14),41(7)
Reference information Reference 1
User's Publication 0


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Reference
993  Takaoka T, Yasumoto S, Katsuta H.  A simple method for the cultivation of rat liver cells.  Jpn J Exp Med  1975  45(5):317-26  PubMed ID: 1223332  

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