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Cell No. : Cell Name
RCB0067 : M  update : 2022/03/04
CommentCapable to form collagen network
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takaoka, Toshiko
Originator Takaoka,T. & Katsuta, H.
Year of deposit 1986
Animal rat < Mammals
Tissue liver
Classification other
Year of origin 1964
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5550
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
991  Katsuta H, Takaoka T.  An attempt in tissue culture at preventing and treating the collagen fiber formation of liver cells.  Jpn J Exp Med  1980  50(4):275-82  PubMed ID: 7441906  
990  H Katsuta  Cytobiological transformation of normal rat liver cells by treatment with 4-dimethylaminoazobenzene after Nagisa culture.  Cancer Cells in Culture  1968  321-334   

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