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Cell No. : Cell Name
RCB0065 : JTC-27  update : 2022/03/04
CommentAscites hepatoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takaoka, Toshiko
Originator Huh,Namho et al.
Year of deposit 1986
Animal rat < Mammals
Tissue ascites hepatoma
Classification cancer
Year of origin 1971
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_2085
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
3666  高岡 聰子,秋元 一三  組織培養におけるエラスターゼの作用  組織培養研究  1984  3:31-32   
989  Huh N, Takaoka T, Katsuta H  Tissue culture of rat ascites hepatoma AH-601 cells.  Jpn J Exp Med  1972  42:249-62  PubMed ID: 4340679  

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