Cell No. : Cell Name
RCB0063 : JTC-15
update : 2023/01/04
|
| Comment | Subclone of AH-66, lost transplantability in rats. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Takaoka, Toshiko
|
| Originator |
Takaoka,T. & Katsuta, H.
|
| Year of deposit |
1986
|
| Another name |
66
|
| Animal |
_rat
< Mammals
|
| Tissue |
ascites hepatoma
|
| Classification |
cancer
|
| Year of origin |
1963
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_2084
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, G6PD, PepB
|
| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
3655
Kondo M, Hirota N, Takaoka T, Kajiwara M.
Heme-biosynthetic enzyme activities and porphyrin accumulation in normal liver and hepatoma cell lines of rat.
Cell Biol Toxicol
1993
9(1):95-105
PubMed ID: 8390914
DOI: 10.1007/bf00755143
|
|
12
Hajim Katsuta, Toshiko Takaoka, Hidetoshi Ito
Tapping culture-an improved method for cell suspension culture
In Vitro
1979
15:949-956
DOI: 10.1007/bf02619154
|
|
147
Takaoka T, Katsuta H, Akatsuka T.
Carcinogenesis in tissue culture 24: tumorigenicity and aggregate-forming capacity of mammalian cells in culture
Jpn J Exp Med
1975
45(4):299-312
PubMed ID: 171466
|
|
13
Katsuta H, Takaoka T, Yasumoto S.
Toxic metabolites released from rat hepatoma cells in culture. I. Effects of metabolites of hepatomas on various cells
J Natl Cancer Inst
1973
51(6):1841-4
PubMed ID: 4358144
DOI: 10.1093/jnci/51.6.1841
|
|
988
Takaoka T, Katsuta H, Ota S, Miyata M.
Two cell strains of tissue culture, JTC-15 and -16, from rat ascites hepatomas, AH-66 and -7974.
Jpn J Exp Med
1969
39(3):267-75
PubMed ID: 4312683
|
Back
Top
CELL BANK Website