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Cell No. : Cell Name
RCB0063 : JTC-15  update : 2023/01/04
CommentSubclone of AH-66, lost transplantability in rats.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takaoka, Toshiko
Originator Takaoka,T. & Katsuta, H.
Year of deposit 1986
Another name 66
Animal rat < Mammals
Tissue ascites hepatoma
Classification cancer
Year of origin 1963
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2084
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, PepB
Reference information Reference 5
User's Publication 0

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3655  Kondo M, Hirota N, Takaoka T, Kajiwara M.  Heme-biosynthetic enzyme activities and porphyrin accumulation in normal liver and hepatoma cell lines of rat.  Cell Biol Toxicol  1993  9(1):95-105  PubMed ID: 8390914   DOI: 10.1007/bf00755143
12  Hajim Katsuta, Toshiko Takaoka, Hidetoshi Ito  Tapping culture-an improved method for cell suspension culture  In Vitro  1979  15:949-956    DOI: 10.1007/bf02619154
147  Takaoka T, Katsuta H, Akatsuka T.  Carcinogenesis in tissue culture 24: tumorigenicity and aggregate-forming capacity of mammalian cells in culture  Jpn J Exp Med  1975  45(4):299-312  PubMed ID: 171466  
13  Katsuta H, Takaoka T, Yasumoto S.  Toxic metabolites released from rat hepatoma cells in culture. I. Effects of metabolites of hepatomas on various cells  J Natl Cancer Inst  1973  51(6):1841-4  PubMed ID: 4358144   DOI: 10.1093/jnci/51.6.1841
988  Takaoka T, Katsuta H, Ota S, Miyata M.  Two cell strains of tissue culture, JTC-15 and -16, from rat ascites hepatomas, AH-66 and -7974.  Jpn J Exp Med  1969  39(3):267-75  PubMed ID: 4312683  

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