Cell No. : Cell Name RCB0056 : NIH3T3/14-1  update : 2023/05/27
Comment			Spontaneous transformant of NIH3T3
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Abe, Shin-ichiro
		Originator	Abe, Shin-ichiro
		Year of deposit	1986
		Animal	_mouse < Mammals
		Strain name	NIH Swiss
		Age at sampling	embryo
		Tissue	embryo
		Classification	other
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_L995
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	2

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Reference

5004  Benveniste RE, Lieber MM, Todaro GJ.  A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.  Proc Natl Acad Sci U S A.  1974  71(3):602-6  PubMed ID: 4132531   DOI: 10.1073/pnas.71.3.602

298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790   DOI: 10.1128/JVI.4.5.549-553.1969

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User's Publication

7670  Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H.  Activity of bone morphogenetic protein-7 after treatment at various temperatures: freezing vs. pasteurization vs. allograft.  Cryobiology  2011  63:235-9  PubMed ID: 21963423   DOI: 10.1016/j.cryobiol.2011.09.001

9009  H Imamura, S Takao, T Aikou  A Modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium Bromide Assay for Quantitating Tumor Cell Invasion  Cancer Res  1994  54 (13), 3620-4  PubMed ID: 8012990