Cell No. : Cell Name
RCB0056 : NIH3T3/14-1
update : 2023/05/27
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Comment | Spontaneous transformant of NIH3T3 |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Abe, Shin-ichiro
|
Originator |
Abe, Shin-ichiro
|
Year of deposit |
1986
|
Animal |
_mouse
< Mammals
|
Strain name |
NIH Swiss
|
Age at sampling |
embryo
|
Tissue |
embryo
|
Classification |
other
|
|
Lifespan |
infinite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_L995
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
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Adherent cells
|
Culture medium |
|
MEM + 10% FBS
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Antibiotics |
|
Free
|
|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
|
SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma/Acholeplasma |
|
(-)
|
SSLP(mouse) |
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OK
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Isozyme |
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LD, NP
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Images |
deposit info | lot info |
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Reference information |
Reference |
2
|
User's Publication |
3
|
User's Publication |
22063
Inoue Y, Yoshino K, Kudo S, Kodama N, Moteki H, Kimura M.
Preparation, solubility, and anti-inflammatory effects of a complex of diphenylcyclopropenone/β-cyclodextrin derivatives as the treatment of alopecia areata.
J Pharm Pharm Sci
2024
27:13230
PubMed ID: 39193564
DOI: 10.3389/jpps.2024.13230
|
7670
Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H.
Activity of bone morphogenetic protein-7 after treatment at various temperatures: freezing vs. pasteurization vs. allograft.
Cryobiology
2011
63:235-9
PubMed ID: 21963423
DOI: 10.1016/j.cryobiol.2011.09.001
|
9009
H Imamura, S Takao, T Aikou
A Modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium Bromide Assay for Quantitating Tumor Cell Invasion
Cancer Res
1994
54 (13), 3620-4
PubMed ID: 8012990
|