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Cell No. : Cell Name
RCB0056 : NIH3T3/14-1  update : 2022/03/04
CommentSpontaneous transformant of NIH3T3
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Abe, Shin-ichiro
Originator Abe, Shin-ichiro
Year of deposit 1986
Animal mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_L995
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 2


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Reference
5004  Benveniste RE, Lieber MM, Todaro GJ.  A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.  Proc Natl Acad Sci U S A.  1974  71(3):602-6  PubMed ID: 4132531   DOI: 10.1073/pnas.71.3.602
298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790  

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User's Publication
7670  Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H.  Activity of bone morphogenetic protein-7 after treatment at various temperatures: freezing vs. pasteurization vs. allograft.  Cryobiology  2011  63:235-9  PubMed ID: 21963423   DOI: 10.1016/j.cryobiol.2011.09.001
9009  H Imamura, S Takao, T Aikou  A Modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium Bromide Assay for Quantitating Tumor Cell Invasion  Cancer Res  1994  54 (13), 3620-4  PubMed ID: 8012990  



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