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細胞番号 : 細胞名
RCB0043 : NRK  update : 2023/06/30
細胞特性(Comment:英)Contact-inhibited semi-normal, original of NRK49F
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Ohno, Tadao
寄託日 1986
動物種 _rat < Mammals
属名 Rattus
種名 norvegicus
採取組織 kidney
細胞分類 semi-normal
入手歴 NIH originator-Osada-RCB
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_3758
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP, G6PD
染色体検査 36-82(50) : 36(1),39(3),40(6),41(30),42(6),43(2)/
文献情報 Reference(英) 2件
Reference(日) 3件
利用者成果(英) 16件
利用者成果(日) 0件

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1807  安光英太郎  ラット:NRK  生体の科学  1992  43:436   
1257  大野忠夫  バイオテクノロジ-素材としての培養細胞 遺伝子移入と発現  蛋白質 核酸 酵素  1988  33:2406   
1564  大野忠夫  NRK  蛋白質 核酸 酵素  1988  33:74   

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