細胞番号 : 細胞名
RCB0043 : NRK
update : 2023/06/30
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細胞特性(Comment:英) | Contact-inhibited semi-normal, original of NRK49F |
細胞特性(日) | NRK49F(RCB0112)のもとの細胞。接触阻止能を示す。 |
細胞特性(寄託者記述:英) | |
細胞特性(寄託者記述:日) | |
使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
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|
寄託者 |
Ohno, Tadao
|
寄託日 |
1986
|
動物種 |
_rat
< Mammals
|
属名 |
Rattus
|
種名 |
norvegicus
|
採取組織 |
kidney
|
細胞分類 |
semi-normal
|
入手歴 |
NIH originator-Osada-RCB
|
|
細胞寿命 |
infinite
|
細胞形態 |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_3758
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細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Adherent cells
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培地 |
|
MEM + 10% FBS
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抗生物質 |
|
Free
|
|
継代方法 |
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0.25% Trypsin
|
|
継代密度 |
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1 : 8 split
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継代・培地交換頻度 |
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Subculture : once/week, Medium Renewal : 2 times/week
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培養最適温度 |
|
37
℃
|
二酸化炭素濃度 |
|
5
%
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凍結培地 |
|
Medium + 10% DMSO
|
凍結方法 |
|
Slow freezing
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マイコプラズマ/アコレプラズマ |
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(-)
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動物種PCR |
|
OK
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アイソザイム検査 |
|
LD, NP, G6PD
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染色体検査 |
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36-82(50) : 36(1),39(3),40(6),41(30),42(6),43(2)/
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画像情報 |
寄託時情報 | ロット情報 |
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文献情報 |
Reference(英) |
2件
|
Reference(日) |
3件
|
利用者成果(英) |
17件
|
利用者成果(日) |
0件
|
利用者成果(英) |
6934
Baba K, Kuwada S, Nakao A, Li X, Okuda N, Nishida A, Mitsuda S, Fukuoka N, Kakeya H, Kataoka T.
Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.
Histochem Cell Biol
2020
PubMed ID: 31907597
DOI: 10.1007/s00418-019-01842-z
|
3919
Tatsunobu Matsui, Hiroshi Ichikawa, Tomoka Fujita, Tomohisa Takagi, Mayuko Osada-Oka, Yukiko Minamiyama
Histidine deficiency attenuates cell viability in rat intestinal epithelial cells by apoptosis via mitochondrial dysfunction
Journal of Nutrition & Intermediary Metabolism
2017
8:21-28
DOI: 10.1016/j.jnim.2017.05.002
|
11685
Miyamoto T, Tamura M, Kabashima N, Serino R, Shibata T, Furuno Y, Miyazaki M, Baba R, Sato N, Doi Y, Okazaki M, Otsuji Y.
An integrin-activating peptide, PHSRN, ameliorates inhibitory effects of conventional peritoneal dialysis fluids on peritoneal wound healing
Nephrol Dial Transplant
2010
25(4):1109-19
PubMed ID: 19926720
DOI: 10.1093/ndt/gfp601
|
8762
Sato Y, Murase K, Kato J, Kobune M, Sato T, Kawano Y, Takimoto R, Takada K, Miyanishi K, Matsunaga T, Takayama T, Niitsu Y.
Resolution of liver cirrhosis using vitamin A-coupled liposomes to deliver siRNA against a collagen-specific chaperone.
Nat. Biotechnol.
2008
26:431-42
PubMed ID: 18376398
DOI: 10.1038/nbt1396
|
2821
Wada K, Mizuno M, Komori T, Tamura M
Extracellular inorganic phosphate regulates gibbon ape leukemia virus receptor-2/phosphate transporter mRNA expression in rat bone marrow stromal cells.
J Cell Physiol
2004
198:40-7
PubMed ID: 14584042
DOI: 10.1002/jcp.10383
|
3009
Fukunaga T, Nagahama M, Hatsuzawa K, Tani K, Yamamoto A, Tagaya M
Implication of sphingolipid metabolism in the stability of the Golgi apparatus
J Cell Sci
2000
113:3299-3307
PubMed ID: 10954427
DOI: 10.1242/jcs.113.18.3299
|
3012
Yamaguchi T, Nagahama M, Itoh H, Hatsuzawa K, Tani K, Tagaya M
Regulation of the golgi structure by the alpha subunits of heterotrimeric G proteins.
FEBS Lett
2000
470:25-8
PubMed ID: 10722839
DOI: 10.1016/s0014-5793(00)01284-9
|
3010
Mashima J, Nagahama M, Hatsuzawa K, Tani K, Horigome T, Yamamoto A, Tagaya M
N-ethylmaleimide-sensitive factor is associated with the nuclear envelope.
Biochem Biophys Res Commun
2000
274:559-64
PubMed ID: 10913377
DOI: 10.1006/bbrc.2000.3162
|
3013
Tani K, Mizoguchi T, Iwamatsu A, Hatsuzawa K, Tagaya M
p125 is a novel mammalian Sec23p-interacting protein with structural similarity to phospholipid-modifying proteins.
J Biol Chem
1999
274:20505-12
PubMed ID: 10400679
DOI: 10.1074/jbc.274.29.20505
|
2364
Nomura R, Fujimoto T
Tyrosine-phosphorylated caveolin-1: immunolocalization and molecular characterization.
Mol Biol Cell
1999
10:975-86
PubMed ID: 10198051
DOI: 10.1091/mbc.10.4.975
|
5179
Kukita A, Kukita T, Ouchida M, Maeda H, Yatsuki H, Kohashi O.
Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis
Blood
1999
94(6):1987-97
PubMed ID: 10477728
|
3014
Fukunaga T, Furuno A, Hatsuzawa K, Tani K, Yamamoto A, Tagaya M
NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus.
FEBS Lett
1998
435:237-40
PubMed ID: 9762917
DOI: 10.1016/s0014-5793(98)01081-3
|
1422
Takahashi S, Takahashi Y, Yoshimi T, Miura T.
Oxygen tension regulates heme oxygenase-1 gene expression in mammalian cell lines
Cell Biochem Funct
1998
16(3):183-93
PubMed ID: 9747510
DOI: 10.1002/(SICI)1099-0844(199809)16:3<183::AID-CBF784>3.0.CO;2-0
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3033
Yamaguchi T, Yamamoto A, Furuno A, Hatsuzawa K, Tani K, Himeno M, Tagaya M
Possible involvement of heterotrimeric G proteins in the organization of the Golgi apparatus.
J Biol Chem
1997
272:25260-6
PubMed ID: 9312142
DOI: 10.1074/jbc.272.40.25260
|
1933
Han K, Hashimoto N, Ikeda Y, Shiratori Y, Kato H, Toda G, Komatsu Y, Yamada H, Tanaka A, Kurokawa K
Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis.
Dig Dis Sci
1995
40:1213-20
PubMed ID: 7781435
DOI: 10.1007/BF02065526
|
9013
T Kukita, A Kukita, K Nagata, H Maeda, K Kurisu, T Watanabe, T Iijima
Novel Cell-Surface Ag Expressed on Rat Osteoclasts Regulating the Function of the Calcitonin Receptor
J Immunol
1994
153 (11), 5265-73
PubMed ID: 7963580
|
1504
Hirano Y, Okuno M, Hayashi T, Goto K, Nakajima A.
Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines
J Biomater Sci Polym Ed
1993
4(3):235-43
PubMed ID: 8476793
DOI: 10.1163/156856293x00546
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