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Cell No. : Cell Name
RCB0043 : NRK  update : 2023/06/30
CommentContact-inhibited semi-normal, original of NRK49F
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Year of deposit 1986
Animal _rat < Mammals
Genus Rattus
Species norvegicus
Tissue kidney
Classification semi-normal
History NIH originator-Osada-RCB
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3758
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD
Chromosome mode 36-82(50) : 36(1),39(3),40(6),41(30),42(6),43(2)/
deposit info
lot info
Reference information Reference 2
User's Publication 16

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300  de Larco, J E, Todaro, G J  Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.  J Cell Physiol  1978  94:335-42  PubMed ID: 304450   DOI: 10.1002/jcp.1040940311
1806  Rosenblum, E N, Zeigel, R F, Zeigel RF  Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus.  J Bacteriol  1966  92:1133-40  PubMed ID: 5926742   DOI: 10.1128/jb.92.4.1133-1140.1966

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User's Publication
6934  Baba K, Kuwada S, Nakao A, Li X, Okuda N, Nishida A, Mitsuda S, Fukuoka N, Kakeya H, Kataoka T.  Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.  Histochem Cell Biol  2020    PubMed ID: 31907597   DOI: 10.1007/s00418-019-01842-z
3919  Tatsunobu Matsui, Hiroshi Ichikawa, Tomoka Fujita, Tomohisa Takagi, Mayuko Osada-Oka, Yukiko Minamiyama  Histidine deficiency attenuates cell viability in rat intestinal epithelial cells by apoptosis via mitochondrial dysfunction  Journal of Nutrition & Intermediary Metabolism  2017  8:21-28    DOI: 10.1016/j.jnim.2017.05.002
11685  Miyamoto T, Tamura M, Kabashima N, Serino R, Shibata T, Furuno Y, Miyazaki M, Baba R, Sato N, Doi Y, Okazaki M, Otsuji Y.  An integrin-activating peptide, PHSRN, ameliorates inhibitory effects of conventional peritoneal dialysis fluids on peritoneal wound healing  Nephrol Dial Transplant  2010  25(4):1109-19  PubMed ID: 19926720   DOI: 10.1093/ndt/gfp601
8762  Sato Y, Murase K, Kato J, Kobune M, Sato T, Kawano Y, Takimoto R, Takada K, Miyanishi K, Matsunaga T, Takayama T, Niitsu Y.  Resolution of liver cirrhosis using vitamin A-coupled liposomes to deliver siRNA against a collagen-specific chaperone.  Nat. Biotechnol.  2008  26:431-42  PubMed ID: 18376398   DOI: 10.1038/nbt1396
2821  Wada K, Mizuno M, Komori T, Tamura M  Extracellular inorganic phosphate regulates gibbon ape leukemia virus receptor-2/phosphate transporter mRNA expression in rat bone marrow stromal cells.  J Cell Physiol  2004  198:40-7  PubMed ID: 14584042   DOI: 10.1002/jcp.10383
3009  Fukunaga T, Nagahama M, Hatsuzawa K, Tani K, Yamamoto A, Tagaya M  Implication of sphingolipid metabolism in the stability of the Golgi apparatus  J Cell Sci  2000  113:3299-3307  PubMed ID: 10954427   DOI: 10.1242/jcs.113.18.3299
3012  Yamaguchi T, Nagahama M, Itoh H, Hatsuzawa K, Tani K, Tagaya M  Regulation of the golgi structure by the alpha subunits of heterotrimeric G proteins.  FEBS Lett  2000  470:25-8  PubMed ID: 10722839   DOI: 10.1016/s0014-5793(00)01284-9
3010  Mashima J, Nagahama M, Hatsuzawa K, Tani K, Horigome T, Yamamoto A, Tagaya M  N-ethylmaleimide-sensitive factor is associated with the nuclear envelope.  Biochem Biophys Res Commun  2000  274:559-64  PubMed ID: 10913377   DOI: 10.1006/bbrc.2000.3162
3013  Tani K, Mizoguchi T, Iwamatsu A, Hatsuzawa K, Tagaya M  p125 is a novel mammalian Sec23p-interacting protein with structural similarity to phospholipid-modifying proteins.  J Biol Chem  1999  274:20505-12  PubMed ID: 10400679   DOI: 10.1074/jbc.274.29.20505
2364  Nomura R, Fujimoto T  Tyrosine-phosphorylated caveolin-1: immunolocalization and molecular characterization.  Mol Biol Cell  1999  10:975-86  PubMed ID: 10198051   DOI: 10.1091/mbc.10.4.975
5179  Kukita A, Kukita T, Ouchida M, Maeda H, Yatsuki H, Kohashi O.  Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis  Blood  1999  94(6):1987-97  PubMed ID: 10477728  
3014  Fukunaga T, Furuno A, Hatsuzawa K, Tani K, Yamamoto A, Tagaya M  NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus.  FEBS Lett  1998  435:237-40  PubMed ID: 9762917   DOI: 10.1016/s0014-5793(98)01081-3
3033  Yamaguchi T, Yamamoto A, Furuno A, Hatsuzawa K, Tani K, Himeno M, Tagaya M  Possible involvement of heterotrimeric G proteins in the organization of the Golgi apparatus.  J Biol Chem  1997  272:25260-6  PubMed ID: 9312142   DOI: 10.1074/jbc.272.40.25260
1933  Han K, Hashimoto N, Ikeda Y, Shiratori Y, Kato H, Toda G, Komatsu Y, Yamada H, Tanaka A, Kurokawa K  Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis.  Dig Dis Sci  1995  40:1213-20  PubMed ID: 7781435   DOI: 10.1007/BF02065526
9013  T Kukita, A Kukita, K Nagata, H Maeda, K Kurisu, T Watanabe, T Iijima  Novel Cell-Surface Ag Expressed on Rat Osteoclasts Regulating the Function of the Calcitonin Receptor  J Immunol  1994  153 (11), 5265-73  PubMed ID: 7963580  
1504  Hirano Y, Okuno M, Hayashi T, Goto K, Nakajima A.  Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines  J Biomater Sci Polym Ed  1993  4(3):235-43  PubMed ID: 8476793   DOI: 10.1163/156856293x00546

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