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Cell No. : Cell Name
RCB0036 : C127  update : 2018/07/06
CommentHost for bovine papiloma virus
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Noda, Makoto
Year of deposit 1986
Another name C127I
Animal mouse < Mammals
Gender Female
Tissue mammary
Case history mammary tumor
Classification other
History Kobe U.-M. Kitagawa
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 5% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Chromosome mode 85-91(50) : /86(4),87(7),88(11),89(12),90(10),91(4)
Others Slow attachment after thawing.
deposit info
lot info
Reference information Reference 1
User's Publication 0

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943  Lowy, D R, Rands E, Scolnick, E M  Helper-independent transformation by unintegrated Harvey sarcoma virus DNA.  J Virol  1978  26:291-8  PubMed ID: 26810

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