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Cell No. : Cell Name
RCB0035 : WEHI-3  update : 2024/01/17
CommentMouse cell line derived from leukemia. Secretes cytokines for hematopoietic cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kanazawa, Satoshi
Year of deposit 1987
Another name W3(H)
Animal _mouse < Mammals
Strain name BALB/c
Tissue blood
Disease name leukemia
Classification other
History Hirosima Univ.- Yanagihara
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_3622
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 13
User's Publication 29

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922  Almeida JL, Dakic A, Kindig K, Kone M, Letham DLD, Langdon S, Peat R, Holding-Pillai J, Hall EM, Ladd M, Shaffer MD, Berg H, Li J, Wigger G, Lund S, Steffen CR, Fransway BB, Geraghty B, Natoli M, Bauer B, Gollin SM, Lewis DW, Reid Y.  Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines  PLoS One  2019  14(6):e0218412  PubMed ID: 31220119   DOI: 10.1371/journal.pone.0218412
143  Walker EB, Lanier LL, Warner NL.  Concomitant induction of the cell surface expression of Ia determinants and accessory cell function by a murine macrophage tumor cell line  J Exp Med  1982  155(2):629-34  PubMed ID: 6173461   DOI: 10.1084/jem.155.2.629
92  Walker EB, Lanier LL, Warner NL.  Characterization and functional properties of tumor cell lines in accessory cell replacement assays  J Immunol  1982  128(2):852-9  PubMed ID: 7033383  
5287  McKean DJ, Infante AJ, Nilson A, Kimoto M, Fathman CG, Walker E, Warner N.  Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells.  J Exp Med  1981  154(5):1419-31  PubMed ID: 6170720   DOI: 10.1084/jem.154.5.1419
17  Baca OG, Akporiaye ET, Aragon AS, Martinez IL, Robles MV, Warner NL.  Fate of phase I and phase II Coxiella burnetii in several macrophage-like tumor cell lines  Infect Immun  1981  33(1):258-66  PubMed ID: 7263063   DOI: 10.1128/iai.33.1.258-266.1981
980  Metcalf D  Clonal extinction of myelomonocytic leukemic cells by serum from mice injected with endotoxin.  Int J Cancer  1980  25:225-33  PubMed ID: 6967053   DOI: 10.1002/ijc.2910250210
1294  Ralph P, Nakoinz I  Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.  Cancer Res  1977  37:546-50  PubMed ID: 318922  
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031  
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528
31  Röllinghoff M, Warner NL.  Specificity of in vivo tumor rejection assessed by mixing immune spleen cells with target and unrelated tumor cells  Proc Soc Exp Biol Med  1973  144(3):813-8  PubMed ID: 4543620   DOI: 10.3181/00379727-144-37688
126  Rouse BT, Röllinghoff M, Warner NL.  Tumor immunity to murine plasma cell tumors. II. Essential role of T lymphocytes in immune response  Eur J Immunol  1973  3(4):218-24  PubMed ID: 4541468   DOI: 10.1002/eji.1830030408
5289  Metcalf D, Moore MA, Warner NL.  Colony formation in vitro by myelomonocytic leukemic cells.  J Natl Cancer Inst  1969  43(4):983-1001  PubMed ID: 5259326  
84  Warner NL, Moore MA, Metcalf D.  A transplantable myelomonocytic leukemia in BALB-c mice: cytology, karyotype, and muramidase content  J Natl Cancer Inst  1969  43(4):963-8  PubMed ID: 5259325  

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User's Publication
21235  Lim SM, Fujino T, Kim C, Lee G, Lee YH, Kim DW, Ahn JS, Mitsudomi T, Jin T, Lee SY.  BBT-176, a Novel Fourth-Generation Tyrosine Kinase Inhibitor for Osimertinib-Resistant Epidermal Growth Factor Receptor Mutations in Non-Small Cell Lung Cancer.  Clin Cancer Res  2023    PubMed ID: 37249619   DOI: 10.1158/1078-0432.CCR-22-3901
16770  Fujino T, Suda K, Koga T, Hamada A, Ohara S, Chiba M, Shimoji M, Takemoto T, Soh J, Mitsudomi T.  Foretinib can overcome common on-target resistance mutations after capmatinib/tepotinib treatment in NSCLCs with MET exon 14 skipping mutation  J Hematol Oncol  2022  15(1):79  PubMed ID: 35690785   DOI: 10.1186/s13045-022-01299-z
17935  Saito Y, Koya J, Araki M, Kogure Y, Shingaki S, Tabata M, McClure MB, Yoshifuji K, Matsumoto S, Isaka Y, Tanaka H, Kanai T, Miyano S, Shiraishi Y, Okuno Y, Kataoka K.  Landscape and function of multiple mutations within individual oncogenes  Nature  2020  582(7810):95-99  PubMed ID: 32494066   DOI: 10.1038/s41586-020-2175-2
11561  Yamada R, Fukumoto R, Noyama C, Fujisawa A, Oka S, Imamura T.  An epidermis-permeable dipeptide is a potential cosmetic ingredient with partial agonist/antagonist activity toward fibroblast growth factor receptors.  J Cosmet Dermatol  2020  19(2):477-484  PubMed ID: 31099492   DOI: 10.1111/jocd.12997
12035  Kitao A, Kawamoto S, Kurata K, Hayakawa I, Yamasaki T, Matsuoka H, Sumi Y, Kakeji Y, Kamesaki T, Minami H.  Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.  Int. J. Hematol.  2020    PubMed ID: 31997080   DOI: 10.1007/s12185-020-02831-y
11622  Fujino T, Kobayashi Y, Suda K, Koga T, Nishino M, Ohara S, Chiba M, Shimoji M, Tomizawa K, Takemoto T, Mitsudomi T.  Sensitivity and Resistance of MET Exon 14 Mutations in Lung Cancer to Eight MET Tyrosine Kinase Inhibitors In Vitro.  J Thorac Oncol  2019  14(10):1753-1765  PubMed ID: 31279006   DOI: 10.1016/j.jtho.2019.06.023
17202  Elbadry MI, Mizumaki H, Hosokawa K, Espinoza JL, Nakagawa N, Chonabayashi K, Yoshida Y, Katagiri T, Hosomichi K, Zaimoku Y, Imi T, Nguyen MAT, Fujii Y, Tajima A, Ogawa S, Takenaka K, Akashi K, Nakao S.  Escape hematopoiesis by HLA-B5401-lacking hematopoietic stem progenitor cells in men with acquired aplastic anemia  Haematologica  2019  104(10):e447-e450  PubMed ID: 30890597   DOI: 10.3324/haematol.2018.210856
11331  Nishino M, Suda K, Kobayashi Y, Ohara S, Fujino T, Koga T, Chiba M, Shimoji M, Tomizawa K, Takemoto T, Mitsudomi T.  Effects of secondary EGFR mutations on resistance against upfront osimertinib in cells with EGFR-activating mutations in vitro.  Lung Cancer  2018    PubMed ID: 30527179   DOI: 10.1016/j.lungcan.2018.10.026
4860  Ishibashi T, Yaguchi A, Terada K, Ueno-Yokohata H, Tomita O, Iijima K, Kobayashi K, Okita H, Fujimura J, Ohki K, Shimizu T, Kiyokawa N.  Ph-like ALL-related novel fusion kinase ATF7IP-PDGFRB exhibits high sensitivity to tyrosine kinase inhibitors in murine cells.  Exp Hematol  2016  44(3):177-88.e5  PubMed ID: 26703895   DOI: 10.1016/j.exphem.2015.11.009
15120  Kobayashi Y, Togashi Y, Yatabe Y, Mizuuchi H, Jangchul P, Kondo C, Shimoji M, Sato K, Suda K, Tomizawa K, Takemoto T, Hida T, Nishio K, Mitsudomi T.  EGFR Exon 18 Mutations in Lung Cancer: Molecular Predictors of Augmented Sensitivity to Afatinib or Neratinib as Compared with First- or Third-Generation TKIs  Clin Cancer Res  2015  21(23):5305-13  PubMed ID: 26206867   DOI: 10.1158/1078-0432.CCR-15-1046
18280  Oh B, Kim TY, Min HJ, Kim M, Kang MS, Huh JY, Kim Y, Lee DS.  Synergistic killing effect of imatinib and simvastatin on imatinib-resistant chronic myelogenous leukemia cells  Anticancer Drugs  2013  24(1):20-31  PubMed ID: 23075630   DOI: 10.1097/CAD.0b013e32835a0fbd
8857  Tao Y, Nomura M, Kitabatake N, Tani F.  Mouse CD40-transfected cell lines cannot exhibit the binding and RANTES-stimulating activity of exogenous heat shock protein 70.  Mol. Immunol.  2007  44:1262-73  PubMed ID: 16930707   DOI: 10.1016/j.molimm.2006.06.002
18674  Yamaguchi M, Fujimura K, Toga H, Khwaja A, Okamura N, Chopra R.  Shwachman-Diamond syndrome is not necessary for the terminal maturation of neutrophils but is important for maintaining viability of granulocyte precursors  Exp Hematol  2007  35(4):579-86  PubMed ID: 17379068   DOI: 10.1016/j.exphem.2006.12.010
18761  Nishiyama T, Mishima K, Obara K, Inoue H, Doi T, Kondo S, Saka M, Tabunoki Y, Hattori Y, Kodama T, Tsubota K, Saito I.  Amelioration of lacrimal gland inflammation by oral administration of K-13182 in Sjögren's syndrome model mice  Clin Exp Immunol  2007  149(3):586-95  PubMed ID: 17614971   DOI: 10.1111/j.1365-2249.2007.03448.x
2559  Shimizu A, Tada K, Shukunami C, Hiraki Y, Kurokawa T, Magane N, Kurokawa-Seo M  A novel alternatively spliced fibroblast growth factor receptor 3 isoform lacking the acid box domain is expressed during chondrogenic differentiation of ATDC5 cells.  J Biol Chem  2001  276:11031-40  PubMed ID: 11134040   DOI: 10.1074/jbc.M003535200
2473  Tanaka I, Ishihara H  Enhanced expression of the early retrotransposon in C3H mouse-derived myeloid leukemia cells.  Virology  2001  280:107-14  PubMed ID: 11162824   DOI: 10.1006/viro.2000.0732
2560  Ito A, Kataoka, T R, Kim, D K, Koma Y, Lee, Y M, Kitamura Y  Inhibitory effect on natural killer activity of microphthalmia transcription factor encoded by the mutant mi allele of mice.  Blood  2001  97:2075-83  PubMed ID: 11264174   DOI: 10.1182/blood.v97.7.2075
4871  Feng R, Kabayama A, Uchida K, Hoshino H, Miwa M.  Cell-free entry of human T-cell leukemia virus type 1 to mouse cells.  Jpn J Cancer Res  2001  92(4):410-6  PubMed ID: 11346463   DOI: 10.1111/j.1349-7006.2001.tb01110.x
16915  Sun W, Hattori N, Mutai H, Toyoshima Y, Kimura H, Tanaka S, Shiota K.  PAL31, a nuclear protein required for progression to the S phase  Biochem Biophys Res Commun   2001  280(4):1048-54  PubMed ID: 11162633   DOI: 10.1006/bbrc.2000.4244
2951  Yokota S, Kayano T, Ohta T, Kurimoto M, Yanagi H, Yura T, Kubota H  Proteasome-dependent degradation of cytosolic chaperonin CCT  Biochemical and biophysical research communications  2000  279:712-717  PubMed ID: 11118350   DOI: 10.1006/bbrc.2000.4011
2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265   DOI: 10.1074/jbc.274.52.37070
4848  Yoneda A, Asada M, Suzuki M, Imamura T.  Introduction of an N-glycosylation cassette into proteins at random sites: expression of neoglycosylated FGF.  Biotechniques  1999  27(3):576-8  PubMed ID: 10489617   DOI: 10.2144/99273rr05
2215  Saneyoshi K, Nohara O, Imai T, Shiraishi F, Moriyama H, Fujimaki H  IL-4 and IL-6 production of bone marrow-derived mast cells is enhanced by treatment with environmental pollutants.  Int Arch Allergy Immunol  1997  114:237-45  PubMed ID: 9363904   DOI: 10.1159/000237674
18023  Ogasawara T, Murakami M, Suzuki-Nishimura T, Uchida MK, Kudo I.  Mouse bone marrow-derived mast cells undergo exocytosis, prostanoid generation, and cytokine expression in response to G protein-activating polybasic compounds after coculture with fibroblasts in the presence of c-kit ligand  J Immunol  1997  158(1):393-404  PubMed ID: 8977215  
4830  Sasaki M, Sasaki T, Enami J.  Prolactin-dependent growth and gamma-casein gene expression in Ba/F3 cells transfected with a long form of mouse mammary prolactin receptor.  Endocr J  1996  43(1):45-52  PubMed ID: 8732451   DOI: 10.1507/endocrj.43.45
2216  Fujimaki H, Nohara O, Katayama N, Abe T, Nohara K  Ganglioside GM3 inhibits interleukin-3-dependent bone marrow-derived mast cell proliferation.  Int Arch Allergy Immunol  1995  107:527-32  PubMed ID: 7620368   DOI: 10.1159/000237095
5411  Nakamura M, Sakanaka C, Aoki Y, Ogasawara H, Tsuji T, Kodama H, Matsumoto T, Shimizu T, Noma M.  Identification of two isoforms of mouse neuropeptide Y-Y1 receptor generated by alternative splicing. Isolation, genomic structure, and functional expression of the receptors.  J Biol Chem  1995  270(50):30102-10  PubMed ID: 8530415   DOI: 10.1074/jbc.270.50.30102
1730  Katayama, N., Honda, Y. and Fujimaki, H.  Growth and Functional Modifications in Formaldehyde-Treated Mouse Bone Marrow-Derived Mast Cells  Toxicol in Vitro  1992  6:239-243  PubMed ID: 20732119   DOI: 10.1016/0887-2333(92)90037-r
1731  Fujimaki H, Kawagoe A, Bissonnette E, Befus D  Mast cell response to formaldehyde. 1. Modulation of mediator release.  Int Arch Allergy Immunol  1992  98:324-31  PubMed ID: 1384864   DOI: 10.1159/000236206

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