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Cell No. : Cell Name
RCB0034 : FM3A ts T244  update : 2024/01/17
CommentTemperature-sensitive at DNA polymerase
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hanaoka, Fumio
Originator Tsai, Yan-Jyu et al.
Year of deposit 1986
Another name ts T244
Animal _mouse < Mammals
Strain name C3H
Gender Female
Tissue mammary
Disease name mammary carcinoma
Classification mutant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U141
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% CS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 60-166(50) : /69(4),70(3),71(5),72(6),73(17),74(1)/
deposit info
lot info
Reference information Reference 3
User's Publication 0

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959  Tsai, Y J, Hanaoka F, Nakano, M M, Yamada M  A mammalian DNA- mutant decreasing nuclear DNA polymerase alpha activity at nonpermissive temperature.  Biochem Biophys Res Commun  1979  91:1190-5  PubMed ID: 230835   DOI: 10.1016/0006-291x(79)92005-9
118  Nakano N.  Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium  Tohoku J Exp Med  1966  88(1):69-84  PubMed ID: 5920633   DOI: 10.1620/tjem.88.69
119  Yamane I, Nakano N.  Long-term culture of the ascites form of a mouse mammary tumor in serum-free media  Tohoku J Exp Med  1966  88(3):203-14  PubMed ID: 5956504  

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