Cell No. : Cell Name
RCB0033 : FM3A tscl.T85 Tsai
update : 2024/01/17
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| Comment | Temperature-sensitive at chromosome condensation |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Hanaoka, Fumio
|
| Originator |
Mita, Siroo et al.
|
| Year of deposit |
1986
|
| Another name |
ts85
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Gender |
Female
|
| Tissue |
mammary
|
| Disease name |
mammary carcinoma
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| Classification |
cancer
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_4201
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% CS
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| Antibiotics |
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Free
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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33
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
|
OK
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| Isozyme |
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LD, NP, G6PD
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| Chromosome mode |
|
35-44(50) : /36(1),39(3),40(7),41(13),42(9),43(14)/
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
1
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| Reference |
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120
Yasuda H, Matsumoto Y, Mita S, Marunouchi T, Yamada M.
A mouse temperature-sensitive mutant defective in H1 histone phosphorylation is defective in deoxyribonucleic acid synthesis and chromosome condensation
Biochemistry
1981
20(15):4414-9
PubMed ID: 7284331
DOI: 10.1021/bi00518a028
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941
Mita S, Yasuda H, Marunouchi T, Ishiko S, Yamada M.
A temperature-sensitive mutant of cultured mouse cells defective in chromosome condensation.
Exp Cell Res
1980
126(2):407-16
PubMed ID: 6153988
DOI: 10.1016/0014-4827(80)90280-3
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118
Nakano N.
Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium
Tohoku J Exp Med
1966
88(1):69-84
PubMed ID: 5920633
DOI: 10.1620/tjem.88.69
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119
Yamane I, Nakano N.
Long-term culture of the ascites form of a mouse mammary tumor in serum-free media
Tohoku J Exp Med
1966
88(3):203-14
PubMed ID: 5956504
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