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Cell No. : Cell Name
RCB0033 : FM3A tscl.T85 Tsai  update : 2022/02/28
CommentTemperature-sensitive at chromosome condensation
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hanaoka, Fumio
Originator Mita, Siroo et al.
Year of deposit 1986
Another name ts85
Animal _mouse < Mammals
Strain name C3H
Gender Female
Tissue mammary
Disease name mammary carcinoma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4201
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% CS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Chromosome mode 35-44(50) : /36(1),39(3),40(7),41(13),42(9),43(14)/
deposit info
lot info
Reference information Reference 4
User's Publication 1

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120  Yasuda H, Matsumoto Y, Mita S, Marunouchi T, Yamada M.  A mouse temperature-sensitive mutant defective in H1 histone phosphorylation is defective in deoxyribonucleic acid synthesis and chromosome condensation  Biochemistry  1981  20(15):4414-9  PubMed ID: 7284331   DOI: 10.1021/bi00518a028
941  Mita S, Yasuda H, Marunouchi T, Ishiko S, Yamada M.  A temperature-sensitive mutant of cultured mouse cells defective in chromosome condensation.  Exp Cell Res  1980  126(2):407-16  PubMed ID: 6153988   DOI: 10.1016/0014-4827(80)90280-3
118  Nakano N.  Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium  Tohoku J Exp Med  1966  88(1):69-84  PubMed ID: 5920633   DOI: 10.1620/tjem.88.69
119  Yamane I, Nakano N.  Long-term culture of the ascites form of a mouse mammary tumor in serum-free media  Tohoku J Exp Med  1966  88(3):203-14  PubMed ID: 5956504  

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User's Publication
4388  Maehama T, Kawahara K, Nishio M, Suzuki A, Hanada K.  Nucleolar stress induces ubiquitination-independent proteasomal degradation of PICT1 protein.  J Biol Chem  2014  289(30):20802-12  PubMed ID: 24923447   DOI: 10.1074/jbc.M114.571893

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