Cell No. : Cell Name
RCB0032 : FM3A FT-101
update : 2024/01/17
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| Comment | Temperature-sensitive, forms multiple nuclei |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Hanaoka, Fumio
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| Originator |
Yasumitsu, Hidetaro et al.
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| Year of deposit |
1986
|
| Another name |
tsFT101
|
| Animal |
_mouse
< Mammals
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| Strain name |
C3H
|
| Gender |
Female
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| Tissue |
mammary
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| Disease name |
mammary carcinoma
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| Classification |
mutant
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_J378
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% CS
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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33
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Chromosome mode |
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61-182(50) : /70(4),71(5),72(2),73(14),74(3),75(2)/
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
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| User's Publication |
2
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| Reference |
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17475
Kazama H, Kashiwaba SI, Ishii S, Yoshida K, Yatsuo Y, Naraoka T, Fukuoka M, Murakami Y.
Loss of DIAPH3, a Formin Family Protein, Leads to Cytokinetic Failure Only under High Temperature Conditions in Mouse FM3A Cells
Int J Mol Sci
2020
21(22):8493
PubMed ID: 33187357
DOI: 10.3390/ijms21228493
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958
Yasumitsu H, Hanaoka F, Yasuda H, Murakami Y, Enomoto T, Yamada M
Isolation and initial characterization of a temperature-sensitive mutant of mouse FM3A cells defective in cytokinesis.
Cell Struct Funct
1985
10:79-88
PubMed ID: 2581712
DOI: 10.1247/csf.10.79
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118
Nakano N.
Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium
Tohoku J Exp Med
1966
88(1):69-84
PubMed ID: 5920633
DOI: 10.1620/tjem.88.69
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119
Yamane I, Nakano N.
Long-term culture of the ascites form of a mouse mammary tumor in serum-free media
Tohoku J Exp Med
1966
88(3):203-14
PubMed ID: 5956504
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