Back Back
Cell No. : Cell Name
RCB0032 : FM3A FT-101  update : 2024/01/17
CommentTemperature-sensitive, forms multiple nuclei
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hanaoka, Fumio
Originator Yasumitsu, Hidetaro et al.
Year of deposit 1986
Another name tsFT101
Animal _mouse < Mammals
Strain name C3H
Gender Female
Tissue mammary
Disease name mammary carcinoma
Classification mutant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J378
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% CS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 61-182(50) : /70(4),71(5),72(2),73(14),74(3),75(2)/
deposit info
lot info
Reference information Reference 4
User's Publication 1

To topTop
17475  Kazama H, Kashiwaba SI, Ishii S, Yoshida K, Yatsuo Y, Naraoka T, Fukuoka M, Murakami Y.  Loss of DIAPH3, a Formin Family Protein, Leads to Cytokinetic Failure Only under High Temperature Conditions in Mouse FM3A Cells  Int J Mol Sci  2020  21(22):8493  PubMed ID: 33187357   DOI: 10.3390/ijms21228493
958  Yasumitsu H, Hanaoka F, Yasuda H, Murakami Y, Enomoto T, Yamada M  Isolation and initial characterization of a temperature-sensitive mutant of mouse FM3A cells defective in cytokinesis.  Cell Struct Funct  1985  10:79-88  PubMed ID: 2581712   DOI: 10.1247/csf.10.79
118  Nakano N.  Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium  Tohoku J Exp Med  1966  88(1):69-84  PubMed ID: 5920633   DOI: 10.1620/tjem.88.69
119  Yamane I, Nakano N.  Long-term culture of the ascites form of a mouse mammary tumor in serum-free media  Tohoku J Exp Med  1966  88(3):203-14  PubMed ID: 5956504  

To topTop
User's Publication
17474  Cao Y, Motomura K, Ohtsuru A, Matsumoto T, Yamashita S, Kosaka M.  Profilin gene expression and regulation in a temperature-sensitive breast cancer cell line: tsFT101  Pflugers Arch  1997  434(4):341-5  PubMed ID: 9211798   DOI: 10.1007/s004240050406

Back Back Return Top Page