検索画面
細胞材料開発室サイト

戻る 戻る
細胞番号 : 細胞名
RCB0027 : K562  update : 2021/05/06
細胞特性(Comment:英)Human cell line derived from chronic myelogenous leukemia. Sensitive to NK cells. Differentiate to erythroid cells.
細胞特性(日)ヒト慢性骨髄性白血病由来細胞株。ナチュラルキラー細胞に感受性がある。赤血球への分化が可能。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Kanazawa, Satoshi
樹立者 Lozzio, C.B. & Lozzio, B.B.
寄託日 1986
動物種 _human < Mammals
属名 Homo
種名 sapiens
性別 Female
53 years
採取組織 pleural effusion
病名 chronic myelogenous leukemia
細胞分類 cancer
細胞寿命 infinite
細胞形態 lymphoblast-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Suspension cells
培地 HamF12 + 10% FBS
抗生物質 Free
継代方法 dilution
継代密度 1 : 10 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
ウィルス(HIV) (-)
ウィルス(HTLV-1) (-)
アイソザイム検査 LD, NP
染色体検査 59-68(50) : /63(5),64(7),65(6),66(8),67(16),68(5)
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 5件
Reference(日) 1件
利用者成果(英) 56件
利用者成果(日) 0件

トップへページトップへ
Reference(英)
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644
4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658

トップへページトップへ
Reference(日)
1222  角純子  バイオテクノロジ-素材としての培養細胞 細胞分化(I)  蛋白質 核酸 酵素  1988  33 

トップへページトップへ
利用者成果(英)
13403  Takeuchi R, Suzuki M, Yasukawa T.  Electrorotation Rates of K562 Cells Accompanied with the Erythroid Differentiation Induced by Sodium Butyrate.  Anal Sci  2021    PubMed ID: 33390466
20082  Yoshimura A, Asahina Y, Chang LY, Angata T, Tanaka H, Kitajima K, Sato C.  Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells.  J Biol Chem  2021  100477  PubMed ID: 33640457
13052  Kawai S, Suzuki M, Arimoto S, Korenaga T, Yasukawa T.  Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation.  Analyst  2020    PubMed ID: 32462157
13914  Takano M, Higashi M, Baba S, Kawami M, Yumoto R.  Transport of ribavirin in human myelogenous leukemia cell line K562  Pharmazie  2020  75(7):329-334  PubMed ID: 32635975
11537  Miura S, Kobune M, Horiguchi H, Kikuchi S, Iyama S, Murase K, Goto A, Ikeda H, Takada K, Miyanishi K, Kato J.  EPO-R+ myelodysplastic cells with ring sideroblasts produce high erythroferrone levels to reduce hepcidin expression in hepatic cells.  Blood Cells Mol. Dis.  2019    PubMed ID: 31082798
10946  Tsubasa Koga, Keiko Morotomi-Yano, Takashi Sakugawa, Hisato Saitoh, Ken-Ichi Yano  Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells  Sci Rep  2019  9(1):8451  PubMed ID: 31186478
11677  Ryohichi Sugimura, Ryo Ohta, Chihiro Mori, Emi Sano, Tatsuki Sugiyama, Takashi Nagasawa, Akira Niwa,  Angiocrine factors from HUVECs amplify erythroid cells  bioRxiv  2019   
11093  Abdelmoez MN, Iida K, Oguchi Y, Nishikii H, Yokokawa R, Kotera H, Uemura S, Santiago JG, Shintaku H.  SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.  Genome Biol.  2018  19(1):66  PubMed ID: 29871653
4267  Kikuchi G, Kurita R, Ogasawara K, Isa K, Tsuneyama H, Nakamura Y, Yabe R, Shiba M, Tadokoro K, Nagai T, Satake M.  Application of immortalized human erythroid progenitor cell line in serologic tests to detect red blood cell alloantibodies.  Transfusion  2018    PubMed ID: 30180269
4270  Ichii M, Oritani K, Murase M, Komatsu K, Yamazaki M, Kyoden R, Kito N, Nozaki Y, Saito M, Iwamura H, Kanakura Y.  Molecular targeting of inosine-5'-monophosphate dehydrogenase by FF-10501 promotes erythropoiesis via ROS/MAPK pathway.  Leuk. Lymphoma  2018    PubMed ID: 28730859
4378  Miyauchi M, Koya J, Arai S, Yamazaki S, Honda A, Kataoka K, Yoshimi A, Taoka K, Kumano K, Kurokawa M.  ADAM8 Is an Antigen of Tyrosine Kinase Inhibitor-Resistant Chronic Myeloid Leukemia Cells Identified by Patient-Derived Induced Pluripotent Stem Cells.  Stem Cell Reports  2018  10(3):1115-1130  PubMed ID: 29429960
9546  Nakamura A, Monzen S, Takasugi Y, Wojcik A, Mariya Y.  Application of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay.  J. Radiat. Res.  2016  57:121-6  PubMed ID: 26826197
5654  Sadahira Y, Sugihara T, Fujiwara H, Nishimura H, Suetsugu Y, Takeshita M, Okamura S, Goto M.  WRN protein as a novel erythroblast immunohistochemical marker with applications for the diagnosis of Werner syndrome.  Virchows Arch  2015  466(3):343-50  PubMed ID: 25503078
13879  Isoda H, Motojima H, Onaga S, Samet I, Villareal MO, Han J.  Analysis of the erythroid differentiation effect of flavonoid apigenin on K562 human chronic leukemia cells  Chem Biol Interact  2014  220:269-77  PubMed ID: 25058688
9087  Kenta Okamoto, Hitomi Kinoshita, Maria Del Carmen Parquet, Muhareva Raekiansyah, Daisuke Kimura, Katsuyuki Yui, Mohammed Alimul Islam, Futoshi Hasebe, Kouichi Morita  Dengue Virus Strain DEN2 16681 Utilizes a Specific Glycochain of syndecan-2 Proteoglycan as a Receptor  J Gen Virol  2012  93 (Pt 4), 761-770  PubMed ID: 22170634
9088  Haiyan Gong, Kyousuke Kobayashi, Tatsuki Sugi, Hitoshi Takemae, Hitomi Kurokawa, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato  A Novel PAN/apple Domain-Containing Protein From Toxoplasma Gondii: Characterization and Receptor Identification  PLoS One  2012  7 (1), e30169  PubMed ID: 22276154
10993  Soninkhishig Tsolmon, Eri Nakazaki, Junkyu Han, Hiroko Isoda  Apigetrin induces erythroid differentiation of human leukemia cells K562: proteomics approach  Mol Nutr Food Res  2011  55 Suppl 1:S93-S102  PubMed ID: 21480518
12292  Yujiro Tanaka, Koji Kawahashi, Zen-Ichiro Katagiri, Yasuhiro Nakayama, Milind Mahajan, Dimitris Kioussis  Dual function of histone H3 lysine 36 methyltransferase ASH1 in regulation of Hox gene expression  PLoS One  2011  6(11):e28171  PubMed ID: 22140534
9155  Soninkhishig Tsolmon, Junkyu Han, Hiroko Isoda  Inhibition of Cell Growth by Stellera Chamaejasme Extract Is Associated With Induction of Autophagy and Differentiation in Chronic Leukemia K562 Cells  J Biosci Bioeng  2010  110 (2), 262-8  PubMed ID: 20547324
5396  Hosomi O, Misawa Y, Takeya A, Matahira Y, Sugahara K, Kubohara Y, Yamakura F, Kudo S.  Novel oligosaccharide has suppressive activity against human leukemia cell proliferation.  Glycoconj J  2009  26(2):189-98  PubMed ID: 18726690
8795  Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761
3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080
9051  Tominaga K, Kagata T, Johmura Y, Hishida T, Nishizuka M, Imagawa M.  SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc.  FEBS J.  2005  272:1590-9  PubMed ID: 15794747
3170  Harada H, Suzu S, Ito T, Okada S.  Selective expansion and engraftment of human CD16+ NK cells in NOD/SCID mice.  Eur J Immunol  2005  35(12):3599-609  PubMed ID: 16304638
9048  Shiro Okumura, Hiroyuki Saitoh, Tomoyuki Ishikawa, Naoya Wasano, Satoko Yamashita, Ken-Ichi Kusumoto, Tetsuyuki Akao, Eiichi Mizuki, Michio Ohba, Kuniyo Inouye  Identification of a Novel Cytotoxic Protein, Cry45Aa, From Bacillus Thuringiensis A1470 and Its Selective Cytotoxic Activity Against Various Mammalian Cell Lines  J Agric Food Chem  2005  53 (16), 6313-8  PubMed ID: 16076112
2706  Harada H, Watanabe S, Saijo K, Ishiwata I, Ohno T  A Wilms tumor cell line, HFWT, can greatly stimulate proliferation of CD56+ human natural killer cells and their novel precursors in blood mononuclear cells.  Exp Hematol  2004  32:614-21  PubMed ID: 15246157
2848  Feng, Xingmin, Chuhjo, Tatsuya, Sugimori, Chiharu, Kotani, Takeharu, Lu, Xuzhang, Takami, Akiyoshi, Takamatsu, Hiroyuki, Yamazaki, Hirohito, Nakao, Shinji  Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells.  Blood  2004  104:2425-31  PubMed ID: 15217832
2880  Okada M, Adachi S, Imai T, Watanabe K, Toyokuni SY, Ueno M, Zervos AS, Kroemer G, Nakahata T.  A novel mechanism for imatinib mesylate-induced cell death of BCR-ABL-positive human leukemic cells: caspase-independent, necrosis-like programmed cell death mediated by serine protease activity.  Blood  2004  103(6):2299-307  PubMed ID: 14645012
2983  Kawano T, Horiguchi-Yamada J, Iwase S, Akiyama M, Furukawa Y, Kan Y, Yamada H.  Depsipeptide enhances imatinib mesylate-induced apoptosis of Bcr-Abl-positive cells and ectopic expression of cyclin D1, c-Myc or active MEK abrogates this effect.  Anticancer Res  2004  24(5A):2705-12  PubMed ID: 15517875
2984  Kawano T, Horiguchi-Yamada J, Saito S, Iwase S, Furukawa Y, Kano Y, Yamada H.  Ectopic cyclin D1 expression blocks STI571-induced erythroid differentiation of K562 cells.  Leuk Res  2004  28(6):623-9  PubMed ID: 15120940
2985  Kawano T, Horiguchi-Yamada J, Iwase S, Furukawa Y, Kano Y, Yamada H.  Inactivation of ERK accelerates erythroid differentiation of K562 cells induced by herbimycin A and STI571 while activation of MEK1 interferes with it.  Mol Cell Biochem  2004  58(1-2):25-33  PubMed ID: 15030167
3118  Uchiyama S, Kobayashi S, Takata H, Ishihara T, Sone T, Matsunaga S, Fukui K.  Protein composition of human metaphase chromosomes analyzed by two-dimensional electrophoreses.  Cytogenet Genome Res  2004  107(1-2):49-54  PubMed ID: 15305056
9534  Shunji Aoki, Dexin Kong, Kouhei Matsui, Rachmaniar Rachmat, Motomasa Kobayashi  Sesquiterpene Aminoquinones, From a Marine Sponge, Induce Erythroid Differentiation in Human Chronic Myelogenous Leukemia, K562 Cells  Chem Pharm Bull (Tokyo)   2004  52(8):935-7  PubMed ID: 15304984
9559  Shunji Aoki, Dexin Kong, Kouhei Matsui, Motomasa Kobayashi  Erythroid Differentiation in K562 Chronic Myelogenous Cells Induced by Crambescidin 800, a Pentacyclic Guanidine Alkaloid  Anticancer Res  2004  24(4):2325-30.  PubMed ID: 15330179
3979  Matsumoto K, Akao Y, Kobayashi E, Ohguchi K, Ito T, Tanaka T, Iinuma M, Nozawa Y.  Induction of apoptosis by xanthones from mangosteen in human leukemia cell lines.  J Nat Prod  2003  66(8):1124-7  PubMed ID: 12932141
9586  Kazuko Yamazaki, Emiko Yamada, Yoshio Kanaji, Tetsuo Yanagisawa, Yoshiyuki Kato, Kanji Sato, Kazue Takano, Yuji Sakasegawa, Kiyotoshi Kaneko  Stimulation of Cellular Prion Protein Expression by TSH in Human Thyrocytes  Biochem Biophys Res Commun  2003  305(4):1034-9  PubMed ID: 12767934
13868  Kobayashi T, Yamanaka R, Homma J, Tsuchiya N, Yajima N, Yoshida S, Tanaka R.  Tumor mRNA-loaded dendritic cells elicit tumor-specific CD8(+) cytotoxic T cells in patients with malignant glioma  Cancer Immunol Immunother  2003  52(10):632-7  PubMed ID: 12827308
2549  Nagai, Fusako, Hiyoshi, Yasuko, Sugimachi, Kumiko, Tamura, Hiro-Omi  Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines.  Biol Pharm Bull  2002  25:383-5  PubMed ID: 11913539
4885  Sonoyama J, Matsumura I, Ezoe S, Satoh Y, Zhang X, Kataoka Y, Takai E, Mizuki M, Machii T, Wakao H, Kanakura Y.  Functional cooperation among Ras, STAT5, and phosphatidylinositol 3-kinase is required for full oncogenic activities of BCR/ABL in K562 cells.  J Biol Chem  2002  277(10):8076-82  PubMed ID: 11779872
2979  Horiguchi-Yamada J, Fukumi S, Saito S, Nakayama R, Iwase S, Yamada H.  DNA topoisomerase II inhibitor, etoposide, induces p21WAF1/CIP1 through down-regulation of c-Myc in K562 cells.  Anticancer Res  2002  22(6C):3827-32  PubMed ID: 12553001
13865  Jiang B, Hattori N, Liu B, Kitagawa K, Inagaki C.  Expression of swelling- and/or pH-regulated chloride channels (ClC-2, 3, 4 and 5) in human leukemic and normal immune cells  Life Sci  2002  70(12):1383-94  PubMed ID: 11883714
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959
13866  Hattori N, Saito T, Yagyu T, Jiang BH, Kitagawa K, Inagaki C.  GH, GH receptor, GH secretagogue receptor, and ghrelin expression in human T cells, B cells, and neutrophils  J Clin Endocrinol Metab  2001  86(9):4284-91  PubMed ID: 11549663
13876  Aoki S, Higuchi K, Isozumi N, Matsui K, Miyamoto Y, Itoh N, Tanaka K, Kobayashi M.  Differentiation in chronic myelogenous leukemia cell K562 by spongean sesterterpene  Biochem Biophys Res Commun  2001  282(2):426-31  PubMed ID: 11401476
2986  Fukumi S, Horiguchi-Yamada J, Iwase S, Ohno T, Yamada H.  Concentration-dependent variable effects of etoposide on the cell cycle of CML cells.  Anticancer Res  2000  20(5A):3105-10  PubMed ID: 11062729
2987  Akiyama M, Yamada O, Akita S, Urashima M, Horiguchi-Yamada J, Ohno T, Mizoguchi H, Eto Y, Yamada H.  Ectopic expression of c-myc fails to overcome downregulation of telomerase activity induced by herbimycin A, but ectopic hTERT expression overcomes it.  Leukemia  2000  14(7):1260-5  PubMed ID: 10914551
2988  Fukumi S, Horiguchi-Yamada J, Nakada S, Nagai M, Ohno T, Yamada H.  Differential responses of Bcl-2 family genes to etoposide in chronic myeloid leukemia K562 cells.  Mol Cell Biochem  2000  206(1-2):43-50  PubMed ID: 10839193
2381  Suzuki K, Tsujitani S, Konishi I, Yamaguchi Y, Hirooka Y, Kaibara N.  Expression of MAGE genes and survival in patients with hepatocellular carcinoma.  Int J Oncol  1999  15(6):1227-32  PubMed ID: 10568832
2991  Yamada H, Horiguchi-Yamada J, Nagai M, Takahara S, Sekikawa T, Kawano T, Itoh K, Fukumi S, Iwase S.  Biological effects of a relatively low concentration of 1-beta-D-arabinofuranosylcytosine in K562 cells: alterations of the cell cycle, erythroid-differentiation, and apoptosis.  Mol Cell Biochem  1998  187(1-2):211-20  PubMed ID: 9788759
2320  Kim C, Matsumura M, Saijo K, Ohno T.  In vitro induction of HLA-A2402-restricted and carcinoembryonic-antigen-specific cytotoxic T lymphocytes on fixed autologous peripheral blood cells.  Cancer Immunol Immunother.  1998  47(2):90-6  PubMed ID: 9769117
2318  Liu SQ, Kawai K, Shiraiwa H, Hayashi H, Akaza H, Hashizaki K, Shiba R, Saijo K, Ohno T.  High rate of induction of human autologous cytotoxic T lymphocytes against renal carcinoma cells cultured with an interleukin cocktail.  Jpn J Cancer Res  1998  89(11):1195-201  PubMed ID: 9914789
13863  Isoda H, Nakahara T.  Mannosylerythritol lipid induces granulocytic differentiation and inhibits the tyrosine phosphorylation of human myelogenous leukemia cell line K562  Cytotechnology  1997  25(1-3):191-5  PubMed ID: 22358891
2054  Enomoto K, Furuya K, Moore, R C, Yamagishi S, Oka T, Maeno T  Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells  Biological Signals  1996  5:9-21  PubMed ID: 8739319
2989  Yamada H, Iwase S, Nagai M, Nemoto T, Sekikawa T, Takahara S, Nakada S, Furukawa Y, Horiguchi-Yamada J.  Herbimycin A down-regulates messages of cyclin D1 and c-myc during erythroid differentiation of K562 cells.  Int J Hematol  1996  65(1):31-40  PubMed ID: 8990623
13880  Okada M, Sagawa T, Tominaga A, Kodama T, Hitsumoto Y.  Two mechanisms for platelet-mediated killing of tumour cells: one cyclo-oxygenase dependent and the other nitric oxide dependent  Immunology  1996  89(1):158-64  PubMed ID: 8911154
1332  Shionoya S.  Etoposide inhibition of methylaminoisobutyric acid transport and its recovery in K562 cells.  Leuk Res  1991  15(2-3):137-41  PubMed ID: 2016905

トップへページトップへ
利用者成果(日)



戻る 戻る 理研トップページへ