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Cell No. : Cell Name
RCB0026 : PAb1400  update : 2024/01/17
CommentAnti-SV40 large T antigen mAb.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamaguchi, Nobuo
Originator Yamaguchi, Nobuo
Year of deposit 1986
Another name 416
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Year of origin 1981
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4562
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3・NS-1/1・Ag4.1
Fusion_cell_2 strain mouse
Fusion method PEG
Antibody name PAb1400
Subclass IgG1
Immunogen SV40 transformed BALB/c cell extract
Antigen_name SV40 T antigen
Antigen_m.w. 100000
Antigen_character ds DNA binding,transformed cell
Antigen_positive SV40 transformed cell
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium GIT
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium GIT : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
deposit info
lot info
Reference information Reference 0
User's Publication 1

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User's Publication
17943  Takahashi C, Harada Y, Ariga H, Iguchi-Ariga SM.  Involvement of PIM-1 in DNA fragmentation in mouse NS-1-derived cells  Biochem Biophys Res Commun  1995  3;215(2):538-46  PubMed ID: 7487989   DOI: 10.1006/bbrc.1995.2498

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