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Cell No. : Cell Name
RCB0016 : CCRF-HSB-2  update : 2022/02/25
CommentT cell leukemia
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Todokoro, Kazuo
Originator G.E. Foley et al.
Year of deposit 1986
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Disease name T-cell leukemia
Classification cancer
Year of origin 1966
History JCRB
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_1859
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 45-92(50) : 45(3),46(42),47(4),92(1)
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 4

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5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
4601  Adams RA, Flowers A, Davis BJ.  Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2.  Cancer Res  1968  28(6):1121-5.  PubMed ID: 4872716  

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User's Publication
16817  Kasahara Y, Shin C, Kubo N, Mihara K, Iwabuchi H, Takachi T, Imamura M, Saitoh A, Imai C.  Development and characterisation of NKp44-based chimeric antigen receptors that confer T cells with NK cell-like specificity  Clin Transl Immunology  2020  9(7):e1147  PubMed ID: 32670576   DOI: 10.1002/cti2.1147
15551  Hirohisa Matsushita, Hiroaki Kuwabara, Satoko Ishikawa, Masataka Mochizuki  Apoptosis Induced in Human Cell Lines by a Butanol Extract from Chlorophytum comosum Roots  Journal of Health Science  2005  51:341-345    DOI: 10.1248/jhs.51.341
2643  Williams YN, Baba H, Hayashi S, Ikai H, Sugita T, Tanaka S, Miyasaka N, Kubota T.  Dipeptidyl peptidase IV on activated T cells as a target molecule for therapy of rheumatoid arthritis.  Clin. Exp. Immunol.  2003  131:68-74  PubMed ID: 12519388   DOI: 10.1046/j.1365-2249.2003.02020.x
5423  Tanaka K, Hiramoto T, Fukuda T, Miyagawa K.  A novel human rad54 homologue, Rad54B, associates with Rad51.  J Biol Chem  2000  275(34):26316-21  PubMed ID: 10851248   DOI: 10.1074/jbc.M910306199

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