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Cell No. : Cell Name
RCB0012 : M227 update : 2023/06/30 |
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| Comment | Infected with ts mutant of Kirsten sarcoma virus | |||
| Comment from the depositor | ||||
| Terms and conditions | Send APPROVAL Form(C-0006) directly to RCB. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | RCB (Noda, Makoto) | ||
| Year of deposit | 1986 | |||
| Animal | _mouse < Mammals | |||
| Age at sampling | embryo | |||
| Tissue | embryo/fetus, whole | |||
| Classification | transformed | |||
| History | R. H. Bassin - M. Noda | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_VQ59 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM (low glucose) + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 33 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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