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Cell No. : Cell Name
RCB0012 : M227  update : 2022/02/25
CommentInfected with ts mutant of Kirsten sarcoma virus
Comment from the depositor
Terms and conditionsSend APPROVAL Form(C-0006) directly to RCB.
Remarks
approver's address
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Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor RCB (Noda, Makoto)
Year of deposit 1986
Animal mouse < Mammals
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
History R. H. Bassin - M. Noda
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_VQ59
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
1090  Scolnick, E M, Stephenson, J R, Aaronson, S A  Isolation of temperature-sensitive mutants of murine sarcoma virus.  J Virol  1972  10:653-7  PubMed ID: 4117345  

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