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細胞番号 : 細胞名
RCB0005 : BALB/3T3 clone A31  update : 2021/01/08
細胞特性(Comment:英)A subline of BALB/3T3 cell line. Contact inhibition is well observed.
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Ohno, Tadao
樹立者 Aaronson, S.A. & Todaro, G.T.
寄託日 1986
別名 Balb/c 3T3 A31
動物種 _mouse < Mammals
動物系統名 BALB/c
採取組織 embryo
細胞分類 semi-normal
初代培養日 1968
入手歴 HOIKEN-H.Sakiyama,RIKEN-I.Kane
細胞寿命 infinite
細胞形態 fibroblast-like
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% CS
抗生物質 Free
継代方法 0.25% trypsin
播種細胞数 3 x 10^(5) cells/60 mm dish
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
染色体検査 54-189(50) : /83(4),84(6),85(7),86(4),87(2),88(5)/
文献情報 Reference(英) 7件
Reference(日) 5件
利用者成果(英) 22件
利用者成果(日) 0件

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