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細胞番号 : 細胞名
RCB0005 : BALB/3T3 clone A31  update : 2021/01/08
細胞特性(Comment:英)A subline of BALB/3T3 cell line. Contact inhibition is well observed.
細胞特性(日)BALB/3T3細胞の亜株。接触阻止がよくかかる。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Ohno, Tadao
樹立者 Aaronson, S.A. & Todaro, G.T.
寄託日 1986
別名 Balb/c 3T3 A31
動物種 _mouse < Mammals
動物系統名 BALB/c
embryo
採取組織 embryo
細胞分類 semi-normal
初代培養日 1968
入手歴 HOIKEN-H.Sakiyama,RIKEN-I.Kane
細胞寿命 infinite
細胞形態 fibroblast-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% CS
抗生物質 Free
継代方法 0.25% trypsin
播種細胞数 3 x 10^(5) cells/60 mm dish
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
染色体検査 54-189(50) : /83(4),84(6),85(7),86(4),87(2),88(5)/
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 7件
Reference(日) 5件
利用者成果(英) 22件
利用者成果(日) 0件

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Reference(英)
1819  Kakunaga T, Crow, J D  Cell variants showing differential susceptibility to ultraviolet light--induced transformation.  Science  1980  209:505-7  PubMed ID: 7394516
787  Green,H. and Kehinde,O.  Sublines of mouse 3T3 cells that accumulate lipid.  Cell  1974  1:113-116 
15  Kakunaga T  A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.  Int J Cancer  1973  12:463-73  PubMed ID: 4792350
298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790
595  Aaronson, S A, Todaro, G J  Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.  J Cell Physiol  1968  72:141-8  PubMed ID: 4301006
596  Aaronson, S A, Todaro, G J  Basis for the acquisition of malignant potential by mouse cells cultivated in vitro.  Science  1968  162:1024-6  PubMed ID: 4301647
963  Todaro, G. and Green, H.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.  J Cell Biol  1963  17:299-313  PubMed ID: 13985244

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Reference(日)
1820  梅田誠  マウス:3T3  生体の科学  1992  43:439-440 
1265  黒木登志夫  バイオテクノロジー素材としての培養細胞 化学物質によるト ランスホーメーションとプロモーション  蛋白質 核酸 酵素  1989  34:257-268 
1236  吉竹佳乃,西川克三  バイオテクノロジ-素材としての培養細胞 増殖因子  蛋白質 核酸 酵素  1988  33:1229-1230 
1242  達家雅明,角永武夫  バイオテクノロジ-素材としての培養細胞 細胞周期  蛋白質 核酸 酵素  1988  33:1324-1325 
1235  Yakushinji M, Ono M  バイオテクノロジ-素材としての培養細胞 ホルモン・生理活性物質  蛋白質 核酸 酵素  1988  33:1154 

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利用者成果(英)
4573  Hatai M, Horiyama S, Yoshikawa N, Kinoshita E, Kagota S, Shinozuka K, Nakamura K.  trans-2-Pentenal, an Active Compound in Cigarette Smoke, Identified via Its Ability to Form Adducts with Glutathione.  Chem Pharm Bull (Tokyo)  2019  67(9):1000-1005  PubMed ID: 31474722
11721  Koike T, Harada K, Horiuchi S, Kitamura D.  The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets.  Elife  2019    PubMed ID: 31225793
11954  Lee DH, Arisaka Y, Tonegawa A, Kang TW, Tamura A, Yui N.  Cellular Orientation on Repeatedly Stretching Gelatin Hydrogels with Supramolecular Cross-Linkers.  Polymers (Basel)  2019    PubMed ID: 31847323
10113  Onodera A, Yayama K, Morosawa H, Ishii Y, Tsutsumi Y, Kawai Y.  Reduction of calcium flux from the extracellular region and endoplasmic reticulum by amorphous nano-silica particles owing to carboxy group addition on their surface.  Biochem Biophys Rep  2017  9:330-334  PubMed ID: 29114587
11024    Improved efficiency of nanoneedle insertion by modification with a cell-puncturing protein  Japanese Journal of Applied Physics  2017  57 
6582  Haniuda K, Nojima T, Kitamura D.  In Vitro-Induced Germinal Center B Cell Culture System  Methods Mol Biol  2017  1623:125-133  PubMed ID: 28589353
9514  Yoshie S, Ogasawara Y, Ikehata M, Ishii K, Suzuki Y, Wada K, Wake K, Nakasono S, Taki M, Ohkubo C.  Evaluation of biological effects of intermediate frequency magnetic field on differentiation of embryonic stem cell.  Toxicol Rep  2016  3:135-140  PubMed ID: 28959531
5447  Haniuda K, Fukao S, Kodama T, Hasegawa H, Kitamura D.  Autonomous membrane IgE signaling prevents IgE-memory formation.  Nat Immunol  2016  17(9):1109-17  PubMed ID: 27428827
7539  Kaya K., Ikeda K., Kose R., Sakakura Y., Sano T.  On the function of pentadecanoic acid and docosahexaenoic acid during culturing of the thraustochytrid, Aurantiochytriumsp. NB6-3.  J Biochem Microb Technol.  2015  3:1-7 
6445  Sakurai M, Furusawa T, Ikeda M, Hikono H, Shimizu S, Gotoh H, Kobayashi E, Momotani E  Anti-bovine CD34 monoclonal antibody reveals polymorphisms within coding region of the CD34 gene.  Exp Hematol  2006  34(7):905-13  PubMed ID: 16797418
3232  Arakawa, Reijiro, Tamehiro, Norimasa, Nishimaki-Mogami, Tomoko, Ueda, Kazumitsu, Yokoyama, Shinji  Fenofibric acid, an active form of fenofibrate, increases apolipoprotein A-I-mediated high-density lipoprotein biogenesis by enhancing transcription of ATP-binding cassette transporter A1 gene in a liver X receptor-dependent manner.  Arterioscler Thromb Vasc Biol  2005  25:1193-7  PubMed ID: 15790930
8944  Waki R, Gamo S, Bessho M, Hara M.  Patterning cultured cells by visible light illumination with photosensitizers.  J. Biosci. Bioeng.  2005  100:331-4  PubMed ID: 16243285
9042  Mikihito Nakamori, Xinping Fu, Raphael Rousseau, Si-Yi Chen, Xiaoliu Zhang  Destruction of Nonimmunogenic Mammary Tumor Cells by a Fusogenic Oncolytic Herpes Simplex Virus Induces Potent Antitumor Immunity  Mol Ther  2004  9 (5), 658-65  PubMed ID: 15120326
3226  Yamauchi, Yoshio, Abe-Dohmae, Sumiko, Yokoyama, Shinji  Differential regulation of apolipoprotein A-I/ATP binding cassette transporter A1-mediated cholesterol and phospholipid release.  Biochim Biophys Acta  2002  1585:43110  PubMed ID: 12457709
2956  Shibaki T, Suzutani T, Yoshida I, Ogasawara M, Azuma M  Participation of type I interferon in the decreased virulence of the UL13 gene-deleted mutant of herpes simplex virus type 1.  J Interferon Cytokine Res  2001  21:279-85  PubMed ID: 11429158
6656  Taira M, Toguchi MS, Hamada Y, Takahashi J, Itou R, Toyosawa S, Ijyuin N, Okazaki M.  Studies on cytotoxic effect of nickel ions on three cultured fibroblasts.  J Mater Sci Mater Med  2001  12(5):373-6  PubMed ID: 15348274
2484  Taira M, Nakao H, Matsumoto T, Takahashi J  Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts.  Int J Prosthodont  2000  13:311-5  PubMed ID: 11203647
9022  T Suzutani, M Nagamine, T Shibaki, M Ogasawara, I Yoshida, T Daikoku, Y Nishiyama, M Azuma  The Role of the UL41 Gene of Herpes Simplex Virus Type 1 in Evasion of Non-Specific Host Defence Mechanisms During Primary Infection  J Gen Virol  2000  81 (Pt 7), 1763-71  PubMed ID: 10859382
3969  Akiyama H1, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.  Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.  J Biol Chem  1999.  274(45):32192-7  PubMed ID: 10542256
2275  Nishi N, Matsushita O, Yuube K, Miyanaka H, Okabe A, Wada F  Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain.  Proc Natl Acad Sci U S A  1998  95:7018-23  PubMed ID: 9618531
1946  Kanzaki M, Shibata H, Mogami H, Kojima I  Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells.  J Biol Chem  1995  270:13099-104  PubMed ID: 7539422
1943  Takemoto Y, Furuta M, Li, X K, Strong-Sparks, W J, Hashimoto Y  LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck.  EMBO J  1995  14:3403-14  PubMed ID: 7628441

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