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Cell No. : Cell Name
RCB0005 : BALB/3T3 clone A31  update : 2023/06/30
CommentA subline of BALB/3T3 cell line. Contact inhibition is well observed.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Aaronson, S.A. & Todaro, G.T.
Year of deposit 1986
Another name Balb/c 3T3 A31
Animal _mouse < Mammals
Strain name BALB/c
Age at sampling embryo
Tissue embryo
Classification semi-normal
Year of origin 1968
History HOIKEN-H.Sakiyama,RIKEN-I.Kane
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0184
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10 5 cells/60 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 54-189(50) : /83(4),84(6),85(7),86(4),87(2),88(5)/
deposit info
lot info
Reference information Reference 8
User's Publication 48

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1956  Honma M, Mizusawa H, Sasaki K, Hayashi M, Ohno T, Tanaka N, Sofuni T.  Demonstration by DNA fingerprint analysis of genomic instability in mouse BALB 3T3 cells during cell transformation  Mutat Res  1994  304(2):167-79  PubMed ID: 7506359   DOI: 10.1016/0027-5107(94)90208-9
1819  Kakunaga T, Crow, J D  Cell variants showing differential susceptibility to ultraviolet light--induced transformation.  Science  1980  209:505-7  PubMed ID: 7394516   DOI: 10.1126/science.7394516
787  Green,H. and Kehinde,O.  Sublines of mouse 3T3 cells that accumulate lipid.  Cell  1974  1:113-116    DOI: 10.1016/0092-8674(74)90126-3
15  Kakunaga T  A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.  Int J Cancer  1973  12:463-73  PubMed ID: 4792350   DOI: 10.1002/ijc.2910120217
298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790   DOI: 10.1128/JVI.4.5.549-553.1969
595  Aaronson, S A, Todaro, G J  Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.  J Cell Physiol  1968  72:141-8  PubMed ID: 4301006   DOI: 10.1002/jcp.1040720208
596  Aaronson, S A, Todaro, G J  Basis for the acquisition of malignant potential by mouse cells cultivated in vitro.  Science  1968  162:1024-6  PubMed ID: 4301647   DOI: 10.1126/science.162.3857.1024
963  Todaro, G. and Green, H.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.  J Cell Biol  1963  17:299-313  PubMed ID: 13985244   DOI: 10.1083/jcb.17.2.299

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User's Publication
19658  Dechojarassri D, Okada T, Tamura H, Furuike T.  Evaluation of Cytotoxicity of Hyaluronic Acid/Chitosan/Bacterial Cellulose-Based Membrane  Materials (Basel)  2023  16(14):5189  PubMed ID: 37512462   DOI: 10.3390/ma16145189
21198  Lau LM, Kuga M, Sano M, Kato G.  CD4-1 and CD4-2 single positive are two major CD4 lymphocyte subpopulations in ginbuna crucian carp Carassius auratus langsdorfii.  Fish Shellfish Immunol  2023  108785  PubMed ID: 37141958   DOI: 10.1016/j.fsi.2023.108785
21215  Oe T, Dechojarassri D, Kakinoki S, Kawasaki H, Furuike T, Tamura H.  Microwave-Assisted Incorporation of AgNP into Chitosan-Alginate Hydrogels for Antimicrobial Applications.  J Funct Biomater  2023  14(4)  PubMed ID: 37103289   DOI: 10.3390/jfb14040199
17580  Nakai M, Imai K, Hashimoto Y.  Cell viability of fine powders in hybrid resins and ceramic materials for CAD/CAM  Dent Mater J  2022  41(3):495-505.  PubMed ID: 35264544   DOI: 10.4012/dmj.2021-261
20545  Tamura A, Lee DH, Arisaka Y, Kang TW, Yui N.  Post-Cross-Linking of Collagen Hydrogels by Carboxymethylated Polyrotaxanes for Simultaneously Improving Mechanical Strength and Cell Proliferation.  ACS Biomater Sci Eng  2022    PubMed ID: 34994537   DOI: 10.1021/acsbiomaterials.1c01521
20594  Yokoyama T, Imai K, Hashimoto Y.  Comparison of cell viability between mouse-derived ES-D3 cells and Balb/c 3T3 cells using denture-base lining materials.  Dent Mater J  2022    PubMed ID: 35264546   DOI: 10.4012/dmj.2021-302
15503  Takeo Takeda, Masahiro Ogawa, Junya Kitamoto, Takahiro Kyoya, Megumi Terada  High Glucose Enhances Skin Sensitizer-induced NRF2 Activation In Vitro  The Open Toxicology Journal  2021  7:8-17    DOI: 10.2174/1874340402107010008
4573  Hatai M, Horiyama S, Yoshikawa N, Kinoshita E, Kagota S, Shinozuka K, Nakamura K.  trans-2-Pentenal, an Active Compound in Cigarette Smoke, Identified via Its Ability to Form Adducts with Glutathione.  Chem Pharm Bull (Tokyo)  2019  67(9):1000-1005  PubMed ID: 31474722   DOI: 10.1248/cpb.c19-00345
11721  Koike T, Harada K, Horiuchi S, Kitamura D.  The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets.  Elife  2019    PubMed ID: 31225793   DOI: 10.7554/eLife.44245
11954  Lee DH, Arisaka Y, Tonegawa A, Kang TW, Tamura A, Yui N.  Cellular Orientation on Repeatedly Stretching Gelatin Hydrogels with Supramolecular Cross-Linkers.  Polymers (Basel)  2019    PubMed ID: 31847323   DOI: 10.3390/polym11122095
10113  Onodera A, Yayama K, Morosawa H, Ishii Y, Tsutsumi Y, Kawai Y.  Reduction of calcium flux from the extracellular region and endoplasmic reticulum by amorphous nano-silica particles owing to carboxy group addition on their surface.  Biochem Biophys Rep  2017  9:330-334  PubMed ID: 29114587   DOI: 10.1016/j.bbrep.2017.01.014
11024    Improved efficiency of nanoneedle insertion by modification with a cell-puncturing protein  Japanese Journal of Applied Physics  2017  57    DOI: 10.7567/JJAP.57.03EB02
6582  Haniuda K, Nojima T, Kitamura D.  In Vitro-Induced Germinal Center B Cell Culture System  Methods Mol Biol  2017  1623:125-133  PubMed ID: 28589353   DOI: 10.1007/978-1-4939-7095-7_11
9514  Yoshie S, Ogasawara Y, Ikehata M, Ishii K, Suzuki Y, Wada K, Wake K, Nakasono S, Taki M, Ohkubo C.  Evaluation of biological effects of intermediate frequency magnetic field on differentiation of embryonic stem cell.  Toxicol Rep  2016  3:135-140  PubMed ID: 28959531   DOI: 10.1016/j.toxrep.2015.12.012
5447  Haniuda K, Fukao S, Kodama T, Hasegawa H, Kitamura D.  Autonomous membrane IgE signaling prevents IgE-memory formation.  Nat Immunol  2016  17(9):1109-17  PubMed ID: 27428827   DOI: 10.1038/ni.3508
7539  Kaya K., Ikeda K., Kose R., Sakakura Y., Sano T.  On the function of pentadecanoic acid and docosahexaenoic acid during culturing of the thraustochytrid, Aurantiochytriumsp. NB6-3.  J Biochem Microb Technol.  2015  3:1-7    DOI: 10.14312/2053-2482.2015-1
15026  Abe S, Yamamoto K, Kurata M, Abe-Suzuki S, Horii R, Akiyama F, Kitagawa M.  Targeting MCM2 function as a novel strategy for the treatment of highly malignant breast tumors  Oncotarget  2015  6(33):34892-90  PubMed ID: 26430873   DOI: 10.18632/oncotarget.5408
15147  Yamauchi Y, Iwamoto N, Rogers MA, Abe-Dohmae S, Fujimoto T, Chang CC, Ishigami M, Kishimoto T, Kobayashi T, Ueda K, Furukawa K, Chang TY, Yokoyama S.  Deficiency in the Lipid Exporter ABCA1 Impairs Retrograde Sterol Movement and Disrupts Sterol Sensing at the Endoplasmic Reticulum  J Biol Chem  2015  290(39):23464-77  PubMed ID: 26198636   DOI: 10.1074/jbc.M115.662668
18893  Amemiya Y, Kawano K, Matsusaki M, Akashi M, Nakamura N, Nakamura C.  Formation of nanofilms on cell surfaces to improve the insertion efficiency of a nanoneedle into cells  Biochem Biophys Res Commun  2012  420(3):662-5  PubMed ID: 22450311   DOI: 10.1016/j.bbrc.2012.03.062
17472  Ashinuma H, Takiguchi Y, Kitazono S, Kitazono-Saitoh M, Kitamura A, Chiba T, Tada Y, Kurosu K, Sakaida E, Sekine I, Tanabe N, Iwama A, Yokosuka O, Tatsumi K.  Antiproliferative action of metformin in human lung cancer cell lines  Oncol Rep  2012  28(1):8-14  PubMed ID: 22576795   DOI: 10.3892/or.2012.1763
15722  Nojima T, Haniuda K, Moutai T, Matsudaira M, Mizokawa S, Shiratori I, Azuma T, Kitamura D.  In-vitro derived germinal centre B cells differentially generate memory B or plasma cells in vivo  Nat Commun  2011  2:465  PubMed ID: 21897376   DOI: 10.1038/ncomms1475
7398  Yanagisawa S, Zhu Z, Kobayashi I, Uchino K, Tamada Y, Tamura T, Asakura T.  Improving cell-adhesive properties of recombinant Bombyx mori silk by incorporation of collagen or fibronectin derived peptides produced by transgenic silkworms.  Biomacromolecules  2007  8(11):3487-92  PubMed ID: 17939714   DOI: 10.1021/bm700646f
6445  Sakurai M, Furusawa T, Ikeda M, Hikono H, Shimizu S, Gotoh H, Kobayashi E, Momotani E  Anti-bovine CD34 monoclonal antibody reveals polymorphisms within coding region of the CD34 gene.  Exp Hematol  2006  34(7):905-13  PubMed ID: 16797418   DOI: 10.1016/j.exphem.2006.04.011
3232  Arakawa, Reijiro, Tamehiro, Norimasa, Nishimaki-Mogami, Tomoko, Ueda, Kazumitsu, Yokoyama, Shinji  Fenofibric acid, an active form of fenofibrate, increases apolipoprotein A-I-mediated high-density lipoprotein biogenesis by enhancing transcription of ATP-binding cassette transporter A1 gene in a liver X receptor-dependent manner.  Arterioscler Thromb Vasc Biol  2005  25:1193-7  PubMed ID: 15790930   DOI: 10.1161/01.ATV.0000163844.07815.c4
8944  Waki R, Gamo S, Bessho M, Hara M.  Patterning cultured cells by visible light illumination with photosensitizers.  J. Biosci. Bioeng.  2005  100:331-4  PubMed ID: 16243285   DOI: 10.1263/jbb.100.331
15100  Sugimoto T, Takiguchi Y, Kurosu K, Kasahara Y, Tanabe N, Tatsumi K, Hiroshima K, Minamihisamatsu M, Miyamoto T, Kuriyama T.  Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer  Oncol Rep  2005  14(4):823-30  PubMed ID: 16142338  
14420  Okaji Y, Tsuno NH, Kitayama J, Saito S, Takahashi T, Kawai K, Yazawa K, Asakage M, Hori N, Watanabe T, Shibata Y, Takahashi K, Nagawa H.  Vaccination with autologous endothelium inhibits angiogenesis and metastasis of colon cancer through autoimmunity  Cancer Sci  2004  95(1):85-90  PubMed ID: 14720332   DOI: 10.1111/j.1349-7006.2004.tb03175.x
5566  Kawazoe Y, Shiba T, Nakamura R, Mizuno A, Tsutsumi K, Uematsu T, Yamaoka M, Shindoh M, Kohgo T.  Induction of calcification in MC3T3-E1 cells by inorganic polyphosphate  J Dent Res  2004  83(8):613-8  PubMed ID: 15271969   DOI: 10.1177/154405910408300806
19017  Shiba T, Nishimura D, Kawazoe Y, Onodera Y, Tsutsumi K, Nakamura R, Ohshiro M.  Modulation of mitogenic activity of fibroblast growth factors by inorganic polyphosphate  J Biol Chem   2003  278(29):26788-92  PubMed ID: 12740373   DOI: 10.1074/jbc.M303468200
17317  S. Terada, K. Yanagihara, Y. Saitoh, K. Takemura, T. Nishimura, M. Miki, M. Sasaki, H. Yamada  Sericin Protein Derived from Silk Accelerates Proliferation of Mammalian Cells  Animal Cell Technology: Basic & Applied Aspects  2003  429-433    DOI: 10.1007/978-94-017-0726-8_74
3226  Yamauchi, Yoshio, Abe-Dohmae, Sumiko, Yokoyama, Shinji  Differential regulation of apolipoprotein A-I/ATP binding cassette transporter A1-mediated cholesterol and phospholipid release.  Biochim Biophys Acta  2002  1585:43110  PubMed ID: 12457709   DOI: 10.1016/s1388-1981(02)00304-9
2956  Shibaki T, Suzutani T, Yoshida I, Ogasawara M, Azuma M  Participation of type I interferon in the decreased virulence of the UL13 gene-deleted mutant of herpes simplex virus type 1.  J Interferon Cytokine Res  2001  21:279-85  PubMed ID: 11429158   DOI: 10.1089/107999001300177466
6656  Taira M, Toguchi MS, Hamada Y, Takahashi J, Itou R, Toyosawa S, Ijyuin N, Okazaki M.  Studies on cytotoxic effect of nickel ions on three cultured fibroblasts.  J Mater Sci Mater Med  2001  12(5):373-6  PubMed ID: 15348274   DOI: 10.1023/a:1011295115612
2484  Taira M, Nakao H, Matsumoto T, Takahashi J  Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts.  Int J Prosthodont  2000  13:311-5  PubMed ID: 11203647  
9022  T Suzutani, M Nagamine, T Shibaki, M Ogasawara, I Yoshida, T Daikoku, Y Nishiyama, M Azuma  The Role of the UL41 Gene of Herpes Simplex Virus Type 1 in Evasion of Non-Specific Host Defence Mechanisms During Primary Infection  J Gen Virol  2000  81 (Pt 7), 1763-71  PubMed ID: 10859382   DOI: 10.1099/0022-1317-81-7-1763
16571  Nagano T, Itoh N, Ebisutani C, Takatani T, Miyoshi T, Nakanishi T, Tanaka K.  The transport mechanism of metallothionein is different from that of classical NLS-bearing protein  J Cell Physiol  2000  185(3):440-6  PubMed ID: 11056015   DOI: 10.1002/1097-4652(200012)185:3<440::AID-JCP15>3.0.CO;2-N
3969  Akiyama H1, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.  Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.  J Biol Chem  1999.  274(45):32192-7  PubMed ID: 10542256   DOI: 10.1074/jbc.274.45.32192
2379  Maeda T, Hiranuma H, Jikko A  Differential expression of the splicing regulatory factor genes during two-step chemical transformation in a BALB/3T3-derived cell line, MT-5.  Carcinogenesis  1999  20:2341-4  PubMed ID: 10590230   DOI: 10.1093/carcin/20.12.2341
2275  Nishi N, Matsushita O, Yuube K, Miyanaka H, Okabe A, Wada F  Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain.  Proc Natl Acad Sci U S A  1998  95:7018-23  PubMed ID: 9618531   DOI: 10.1073/pnas.95.12.7018
18033  Kanzaki M, Nie L, Shibata H, Kojima I.  Activation of a calcium-permeable cation channel CD20 expressed in Balb/c 3T3 cells by insulin-like growth factor-I  J Biol Chem  1997  272(8):4964-9  PubMed ID: 9030557   DOI: 10.1074/jbc.272.8.4964
18046  Kanzaki M, Lindorfer MA, Garrison JC, Kojima I.  Activation of the calcium-permeable cation channel CD20 by alpha subunits of the Gi protein  J Biol Chem  1997  272(23):14733-9  PubMed ID: 9169438   DOI: 10.1074/jbc.272.23.14733
18076  Suzu S, Kimura F, Matsumoto H, Yamada M, Hashimoto K, Shimamura S, Motoyoshi K.  Identification of binding domains for basic fibroblast growth factor in proteoglycan macrophage colony-stimulating factor  Biochem Biophys Res Commun  1997  230(2):392-7  PubMed ID: 9016790   DOI: 10.1006/bbrc.1996.5968
18079  Hata Ji, Takeo J, Segawa C, Yamashita S.  A cDNA encoding fish fibroblast growth factor-2, which lacks alternative translation initiation  J Biol Chem  1997  272(11):7285-9  PubMed ID: 9054425   DOI: 10.1074/jbc.272.11.7285
1946  Kanzaki M, Shibata H, Mogami H, Kojima I  Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells.  J Biol Chem  1995  270:13099-104  PubMed ID: 7539422   DOI: 10.1074/jbc.270.22.13099
1943  Takemoto Y, Furuta M, Li, X K, Strong-Sparks, W J, Hashimoto Y  LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck.  EMBO J  1995  14:3403-14  PubMed ID: 7628441   DOI: 10.1002/j.1460-2075.1995.tb07346.x
17947  Matsumoto N, Kita K, Kojima S, Yamamoto K, Irimura T, Miyagi M, Tsunasawa S, Toyoshima S.  Lymphocyte isoforms of mouse p50 LSP1, which are phosphorylated in mitogen-activated T cells, are formed through alternative splicing and phosphorylation  J Biochem  1995  118(1):237-43  PubMed ID: 8537319   DOI: 10.1093/oxfordjournals.jbchem.a124885
1953  Ohno T, Itagaki H, Tanaka N, Ono H.  Validation study on five different cytotoxicity assays in Japan-an intermediate report  Toxicol In Vitro  1995  9(4):571-6  PubMed ID: 20650130   DOI: 10.1016/0887-2333(95)00041-6
17918  Suzu S, Kimura F, Yamada M, Yanai N, Kawashima T, Nagata N, Motoyoshi K.  Direct interaction of proteoglycan macrophage colony-stimulating factor and basic fibroblast growth factor  Blood  1994  83(11):3113-9  PubMed ID: 8193348  

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