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Cell No. : Cell Name
RCB0005 : BALB/3T3 clone A31  update : 2021/01/08
CommentA subline of BALB/3T3 cell line. Contact inhibition is well observed.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Originator Aaronson, S.A. & Todaro, G.T.
Year of deposit 1986
Another name Balb/c 3T3 A31
Animal mouse < Mammals
Strain name BALB/c
Age at sampling embryo
Tissue embryo
Classification semi-normal
Year of origin 1968
History HOIKEN-H.Sakiyama,RIKEN-I.Kane
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 54-189(50) : /83(4),84(6),85(7),86(4),87(2),88(5)/
deposit info
lot info
Reference information Reference 7
User's Publication 22

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1819  Kakunaga T, Crow, J D  Cell variants showing differential susceptibility to ultraviolet light--induced transformation.  Science  1980  209:505-7  PubMed ID: 7394516
787  Green,H. and Kehinde,O.  Sublines of mouse 3T3 cells that accumulate lipid.  Cell  1974  1:113-116 
15  Kakunaga T  A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.  Int J Cancer  1973  12:463-73  PubMed ID: 4792350
298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790
595  Aaronson, S A, Todaro, G J  Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.  J Cell Physiol  1968  72:141-8  PubMed ID: 4301006
596  Aaronson, S A, Todaro, G J  Basis for the acquisition of malignant potential by mouse cells cultivated in vitro.  Science  1968  162:1024-6  PubMed ID: 4301647
963  Todaro, G. and Green, H.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.  J Cell Biol  1963  17:299-313  PubMed ID: 13985244

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User's Publication
4573  Hatai M, Horiyama S, Yoshikawa N, Kinoshita E, Kagota S, Shinozuka K, Nakamura K.  trans-2-Pentenal, an Active Compound in Cigarette Smoke, Identified via Its Ability to Form Adducts with Glutathione.  Chem Pharm Bull (Tokyo)  2019  67(9):1000-1005  PubMed ID: 31474722
11721  Koike T, Harada K, Horiuchi S, Kitamura D.  The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets.  Elife  2019    PubMed ID: 31225793
11954  Lee DH, Arisaka Y, Tonegawa A, Kang TW, Tamura A, Yui N.  Cellular Orientation on Repeatedly Stretching Gelatin Hydrogels with Supramolecular Cross-Linkers.  Polymers (Basel)  2019    PubMed ID: 31847323
10113  Onodera A, Yayama K, Morosawa H, Ishii Y, Tsutsumi Y, Kawai Y.  Reduction of calcium flux from the extracellular region and endoplasmic reticulum by amorphous nano-silica particles owing to carboxy group addition on their surface.  Biochem Biophys Rep  2017  9:330-334  PubMed ID: 29114587
11024    Improved efficiency of nanoneedle insertion by modification with a cell-puncturing protein  Japanese Journal of Applied Physics  2017  57 
6582  Haniuda K, Nojima T, Kitamura D.  In Vitro-Induced Germinal Center B Cell Culture System  Methods Mol Biol  2017  1623:125-133  PubMed ID: 28589353
9514  Yoshie S, Ogasawara Y, Ikehata M, Ishii K, Suzuki Y, Wada K, Wake K, Nakasono S, Taki M, Ohkubo C.  Evaluation of biological effects of intermediate frequency magnetic field on differentiation of embryonic stem cell.  Toxicol Rep  2016  3:135-140  PubMed ID: 28959531
5447  Haniuda K, Fukao S, Kodama T, Hasegawa H, Kitamura D.  Autonomous membrane IgE signaling prevents IgE-memory formation.  Nat Immunol  2016  17(9):1109-17  PubMed ID: 27428827
7539  Kaya K., Ikeda K., Kose R., Sakakura Y., Sano T.  On the function of pentadecanoic acid and docosahexaenoic acid during culturing of the thraustochytrid, Aurantiochytriumsp. NB6-3.  J Biochem Microb Technol.  2015  3:1-7 
6445  Sakurai M, Furusawa T, Ikeda M, Hikono H, Shimizu S, Gotoh H, Kobayashi E, Momotani E  Anti-bovine CD34 monoclonal antibody reveals polymorphisms within coding region of the CD34 gene.  Exp Hematol  2006  34(7):905-13  PubMed ID: 16797418
3232  Arakawa, Reijiro, Tamehiro, Norimasa, Nishimaki-Mogami, Tomoko, Ueda, Kazumitsu, Yokoyama, Shinji  Fenofibric acid, an active form of fenofibrate, increases apolipoprotein A-I-mediated high-density lipoprotein biogenesis by enhancing transcription of ATP-binding cassette transporter A1 gene in a liver X receptor-dependent manner.  Arterioscler Thromb Vasc Biol  2005  25:1193-7  PubMed ID: 15790930
8944  Waki R, Gamo S, Bessho M, Hara M.  Patterning cultured cells by visible light illumination with photosensitizers.  J. Biosci. Bioeng.  2005  100:331-4  PubMed ID: 16243285
9042  Mikihito Nakamori, Xinping Fu, Raphael Rousseau, Si-Yi Chen, Xiaoliu Zhang  Destruction of Nonimmunogenic Mammary Tumor Cells by a Fusogenic Oncolytic Herpes Simplex Virus Induces Potent Antitumor Immunity  Mol Ther  2004  9 (5), 658-65  PubMed ID: 15120326
3226  Yamauchi, Yoshio, Abe-Dohmae, Sumiko, Yokoyama, Shinji  Differential regulation of apolipoprotein A-I/ATP binding cassette transporter A1-mediated cholesterol and phospholipid release.  Biochim Biophys Acta  2002  1585:43110  PubMed ID: 12457709
2956  Shibaki T, Suzutani T, Yoshida I, Ogasawara M, Azuma M  Participation of type I interferon in the decreased virulence of the UL13 gene-deleted mutant of herpes simplex virus type 1.  J Interferon Cytokine Res  2001  21:279-85  PubMed ID: 11429158
6656  Taira M, Toguchi MS, Hamada Y, Takahashi J, Itou R, Toyosawa S, Ijyuin N, Okazaki M.  Studies on cytotoxic effect of nickel ions on three cultured fibroblasts.  J Mater Sci Mater Med  2001  12(5):373-6  PubMed ID: 15348274
2484  Taira M, Nakao H, Matsumoto T, Takahashi J  Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts.  Int J Prosthodont  2000  13:311-5  PubMed ID: 11203647
9022  T Suzutani, M Nagamine, T Shibaki, M Ogasawara, I Yoshida, T Daikoku, Y Nishiyama, M Azuma  The Role of the UL41 Gene of Herpes Simplex Virus Type 1 in Evasion of Non-Specific Host Defence Mechanisms During Primary Infection  J Gen Virol  2000  81 (Pt 7), 1763-71  PubMed ID: 10859382
3969  Akiyama H1, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.  Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.  J Biol Chem  1999.  274(45):32192-7  PubMed ID: 10542256
2275  Nishi N, Matsushita O, Yuube K, Miyanaka H, Okabe A, Wada F  Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain.  Proc Natl Acad Sci U S A  1998  95:7018-23  PubMed ID: 9618531
1946  Kanzaki M, Shibata H, Mogami H, Kojima I  Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells.  J Biol Chem  1995  270:13099-104  PubMed ID: 7539422
1943  Takemoto Y, Furuta M, Li, X K, Strong-Sparks, W J, Hashimoto Y  LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck.  EMBO J  1995  14:3403-14  PubMed ID: 7628441

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