Cell No. : Cell Name RCB0002 : ARM193  update : 2024/01/17
Comment			Anti-recA protein mAb.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Shibata, Takehiko
		Originator	Makino, Osamu
		Year of deposit	1985
		Another name	Rh 193-(1), HYBRIDOMA 193-(1)
		Animal	_mouse x mouse < Hybrid
		Tissue	hybridoma
		Classification	hybrid cells
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_B992
	hybridoma	Fusion_cell_1 strain	mouse, BALB/c
		Fusion_cell_2 name	P3-X63-Ag8.653
		Fusion_cell_2 strain	mouse
		Fusion method	HAT selection
		Antibody name	ARM193
		Subclass	IgG2b
		Immunogen	Escherichia coli, recA protein
		Antigen_name	recA protein
		Antigen_m.w.	38kDa
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Antibody subclass		IgG2b
Reference information		Reference	11
		User's Publication	1

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Reference

10  Mikawa T, Lkeda M, Shibata T.  Epitope mapping by region-specified PCR-mutagenesis  Methods Mol Biol  2009  524:305-13  PubMed ID: 19377954

9  Shibata T, Ikeda M.  Epitope mapping by region-specified PCR mutagenesis  Methods Mol Biol  1996  66:233-40  PubMed ID: 8959719   DOI: 10.1385/0-89603-375-9:233

11  Kurumizaka H, Shibata T.  Homologous recognition by RecA protein using non-equivalent three DNA-strand-binding sites  J Biochem  1996  119(2):216-23  PubMed ID: 8882707   DOI: 10.1093/oxfordjournals.jbchem.a021224

6  Ikeda M, Hamano K, Shibata T.  Epitope mapping of anti-recA protein IgGs by region specified polymerase chain reaction mutagenesis  J Biol Chem  1992  267(9):6291-6  PubMed ID: 1372903

8  Shibata T, Ikeda M, Makino O, Ikawa S.  Monoclonal antibodies and mechanistic studies on recA protein  Biochimie  1991  73(2-3):209-17  PubMed ID: 1715758   DOI: 10.1016/0300-9084(91)90204-e

1298  Ikeda M, Makino O, Shibata T  Epitopes and active sites of the RecA protein.  J Biol Chem  1990  265:8948-56  PubMed ID: 1692839

1297  Ikeda M, Makino O, Shibata T  Probing the activation stages of the RecA protein by monoclonal IgGs during the pairing of homologous DNA molecules.  J Biol Chem  1990  265:8957-65  PubMed ID: 2140364

7  Ikawa S, Kamiya N, Shibata T.  Defective homologous pairing and proficient processive unwinding by the recA430 mutant protein and intermediates of homologous pairing by recA protein  J Biol Chem  1989  264(35):21167-76  PubMed ID: 2556397

1065  Makino O, Ikawa S, Shibata Y, Maeda H, Ando T, Shibata T  rec-A protein-promoted recombination reaction consists of two independent processes, homologous matching and processive unwinding. A study involving an anti-rec-A protein-monoclonal IgG.  J Biol Chem  1987  262:12237-46  PubMed ID: 3624254

1064  Makino O, Shibata Y, Maeda H, Shibata T, Ando T  Monoclonal antibodies with specific effects on partial activities of recA protein of Escherichia coli.  J Biol Chem  1985  260:15402-5  PubMed ID: 2933399

5  Shibata T, Makino O, Ikawa S, Ohtani T, Iwabuchi M, Shibata Y, Maeda H, Ando T.  Roles of processive unwinding in recombination reactions promoted by RecA protein of Escherichia coli: a study using a monoclonal antibody  Cold Spring Harb Symp Quant Biol  1984  49:541-51  PubMed ID: 6099253   DOI: 10.1101/sqb.1984.049.01.061

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User's Publication

1567  Ohno, T., Kurita, K., Abe, S., Eimori, N., and Ikawa, Y.  A simple Freezing Medium for Serum-Free Cultured Cells  Cytotechnology  1988  1:257-260  PubMed ID: 22359122   DOI: 10.1007/BF00145029