Cell No. : Cell Name
RCB0002 : ARM193
update : 2024/01/17
|
| Comment | Anti-recA protein mAb. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Shibata, Takehiko
|
| Originator |
Makino, Osamu
|
| Year of deposit |
1985
|
| Another name |
Rh 193-(1), HYBRIDOMA 193-(1)
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_B992
|
|
hybridoma
|
Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_2 name |
P3-X63-Ag8.653
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
HAT selection
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|
Antibody name |
ARM193
|
| Subclass |
IgG2b
|
| Immunogen |
Escherichia coli, recA protein
|
|
Antigen_name |
recA protein
|
| Antigen_m.w. |
38kDa
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG2b
|
| Reference information |
Reference |
11
|
| User's Publication |
1
|
| Reference |
|
10
Mikawa T, Lkeda M, Shibata T.
Epitope mapping by region-specified PCR-mutagenesis
Methods Mol Biol
2009
524:305-13
PubMed ID: 19377954
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|
9
Shibata T, Ikeda M.
Epitope mapping by region-specified PCR mutagenesis
Methods Mol Biol
1996
66:233-40
PubMed ID: 8959719
DOI: 10.1385/0-89603-375-9:233
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11
Kurumizaka H, Shibata T.
Homologous recognition by RecA protein using non-equivalent three DNA-strand-binding sites
J Biochem
1996
119(2):216-23
PubMed ID: 8882707
DOI: 10.1093/oxfordjournals.jbchem.a021224
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6
Ikeda M, Hamano K, Shibata T.
Epitope mapping of anti-recA protein IgGs by region specified polymerase chain reaction mutagenesis
J Biol Chem
1992
267(9):6291-6
PubMed ID: 1372903
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8
Shibata T, Ikeda M, Makino O, Ikawa S.
Monoclonal antibodies and mechanistic studies on recA protein
Biochimie
1991
73(2-3):209-17
PubMed ID: 1715758
DOI: 10.1016/0300-9084(91)90204-e
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|
1298
Ikeda M, Makino O, Shibata T
Epitopes and active sites of the RecA protein.
J Biol Chem
1990
265:8948-56
PubMed ID: 1692839
|
|
1297
Ikeda M, Makino O, Shibata T
Probing the activation stages of the RecA protein by monoclonal IgGs during the pairing of homologous DNA molecules.
J Biol Chem
1990
265:8957-65
PubMed ID: 2140364
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7
Ikawa S, Kamiya N, Shibata T.
Defective homologous pairing and proficient processive unwinding by the recA430 mutant protein and intermediates of homologous pairing by recA protein
J Biol Chem
1989
264(35):21167-76
PubMed ID: 2556397
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1065
Makino O, Ikawa S, Shibata Y, Maeda H, Ando T, Shibata T
rec-A protein-promoted recombination reaction consists of two independent processes, homologous matching and processive unwinding. A study involving an anti-rec-A protein-monoclonal IgG.
J Biol Chem
1987
262:12237-46
PubMed ID: 3624254
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1064
Makino O, Shibata Y, Maeda H, Shibata T, Ando T
Monoclonal antibodies with specific effects on partial activities of recA protein of Escherichia coli.
J Biol Chem
1985
260:15402-5
PubMed ID: 2933399
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5
Shibata T, Makino O, Ikawa S, Ohtani T, Iwabuchi M, Shibata Y, Maeda H, Ando T.
Roles of processive unwinding in recombination reactions promoted by RecA protein of Escherichia coli: a study using a monoclonal antibody
Cold Spring Harb Symp Quant Biol
1984
49:541-51
PubMed ID: 6099253
DOI: 10.1101/sqb.1984.049.01.061
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