Cell No. : Cell Name
HPS5207 :
update : 2025/01/16
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| Comment | Human iPS cell line. HPS5207, HPS5208 were derived from the same person. Cryopreserved by vitrification method. |
| Comment from the depositor | Human iPS cell line derived from monozygotic twins donors. Brothers with HPS5209. HPS5207, HPS5208 are derived from the same person. |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Psychiatry. 2020 ;25(11):2695-2711) designated by the DEPOSITOR is required. |
| Remarks | Use of the material requires approval by institutional review board for research ethics. |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2021
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
50s
|
| Tissue |
peripheral blood mononuclear cell
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
|
| Vector |
pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXWB-EBNA1
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM/HamF12 (with GlutaMAX, without HEPES) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 4ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin
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Culture information
|
Passage ratio |
|
1 : 3-6 split
|
| SC frequency |
|
Subculture : once/3-5 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.5-2.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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