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Cell No. : Cell Name
HPS5141 : H2B-GEZ  update : 2024/06/07
CommentHuman iPS cell line derived from a healthy individual.
Comment from the depositorCan be used to visualize cell nuclei and identify mitotic cells by EGFP fluorescence.
Terms and conditions1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (*Manuscript submitted) designated by the DEPOSITOR is required. 3) Attach Appendix to MTA (C-0065).
RemarksCRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
Order Form Order Form(C-0042.pdf)   MTA(C-0065.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Year of deposit 2022
Original cell 1383D6 (HPS1006)
Animal _human < Mammals
Genus Homo
Species sapiens
Race Asian
Gender Male
Age at sampling 36 years
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method 0.5 mM EDTA/PBS(-)
Coating dish 0.25 ug/cm2 Laminin-511 E8 (iMatrix-511) *Add and mix to the cell suspension at thawing and passaging.
Culture information Passage cell No 4-20x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium STEM-CELLBANKER
Freezing method Slow freezing
Mycoplasma (-)
Others Use 10μM Y-27632 when thawing and passaging. Remove it next day.
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
21498  Koji Nakade, Satomi Tsukamoto, Kenichi Nakashima, Yuri An, Iori Sato, Jingyue Li, Yuzuno Shimoda, Yasuko Hemmi, Yoshihiro Miwa, Yohei Hayashi  Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system  Cell Reports Methods  2023    PubMed ID: 38086384   DOI: 10.1016/j.crmeth.2023.100662

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