Cell No. : Cell Name
HPS5140 : H2B-TEZ
update : 2024/04/12
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| Comment | Human iPS cell line derived from a healthy individual. |
| Comment from the depositor | Can be used to visualize cell nuclei and identify mitotic cells by tdTomato fluorescence. |
| Terms and conditions | 1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (*Manuscript submitted) designated by the DEPOSITOR is required. 3) Attach Appendix to MTA (C-XXXX). |
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0042.pdf)
MTA(Non-profit_for_USA)C-0072.pdf
MTA(Non-profit_for_Other_Countries)C-0073.pdf
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2022
|
| Original cell |
1383D6 (HPS1006)
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Asian
|
| Gender |
Male
|
| Age at sampling |
36 years
|
| Tissue |
peripheral blood
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
tdTomato, LG3/ EF1alpha promoter, Bleomycin (Zeocin) resistant gene
|
| Vector |
pUCtk2x2-H2B-TEZ
|
| Selection |
Zeocin 1 µg/mL
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.5 mM EDTA/PBS(-)
|
| Coating dish |
|
0.25 ug/cm2 Laminin-511 E8 (iMatrix-511) *Add and mix to the cell suspension at thawing and passaging.
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Culture information
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Passage cell No |
|
4-20x10 4 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
STEM-CELLBANKER
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Others |
|
Use 10μM Y-27632 when thawing and passaging. Remove it next day.
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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