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Cell No. : Cell Name
HPS5067 : HiPS-NB1RGB  update : 2026/09/18
CommentHuman iPS cell line derived from a healthy individual.
Comment from the depositorHuman induced pluripotent stem cells (hiPSCs) derived from normal human skin fibroblast.
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Obtain depositor's consent. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature (eLife, Vol. 12, RP89724, 2024) designated by the DEPOSITOR is required.
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cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank
Fax. +81-29-836-9130
Order Form Order Form(C-0042.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). Regarding commercial use or use related to patent applications, please first contact the RIKEN BRC at cellbank.brc@riken.jp.
Basic information Expected time Upon a request we will start preparation. Contact us: cellqa.brc@riken.jp
IPR RIKEN BioResource Research Center (Japan)
Depositor HAYASHI, Yohei
Originator HAYASHI, Yohei
Year of deposit 2021
Original cell NB1RGB (RCB0222)
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 3 days
Tissue skin
Classification iPS
Recombinant recombinant
Exogene OCT3/4, shRNA against p53, L-MYC, LIN28, SOX2, KLF4, EBNA1
Vector pCXLE-hOCT3/4-shp53-F, pCXLE-hUL, pCXLE-hSK
Lifespan infinite
Morphology ES-like
SDS SDS_DMSO(Japanese)
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method 0.5 mM EDTA/PBS(-)
Coating dish 0.5 ug/cm2 Laminin-511 E8 (iMatrix-511)
Culture information Passage cell No 5-20x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium STEM-CELLBANKER
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Others Use 10μM Y-27632 when thawing and passaging. Remove it next day.
STR(human) OK
Reference information Reference 3
User's Publication 0


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Reference
22172  Matsuo-Takasaki M, Kambayashi S, Hemmi Y, Wakabayashi T, Shimizu T, An Y, Ito H, Takeuchi K, Ibuki M, Kawashima T, Masayasu R, Suzuki M, Kawai Y, Umekage M, Kato TM, Noguchi M, Nakade K, Nakamura Y, Nakaishi T, Nishishita N, Tsukahara M, Hayashi Y.  Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.  Elife  2024  12  PubMed ID: 39529479   DOI: 10.7554/eLife.89724
24180  Ge N, Suzuki K, Sato I, Noguchi M, Nakamura Y, Matsuo-Takasaki M, Fujishiro J, Hayashi Y.  Generation of human induced pluripotent stem cell lines derived from patients of cystic biliary atresia.  Hum Cell  2024  38(1):18.  PubMed ID: 39532815   DOI: 10.1007/s13577-024-01147-x
24138  Shimizu T, Matsuo-Takasaki M, Luijkx D, Takami M, Arai Y, Noguchi M, Nakamura Y, Hayata T, Saito MK, Hayashi Y.  Generation of human induced pluripotent stem cell lines derived from four DiGeorge syndrome patients with 22q11.2 deletion.  Stem Cell Res  2022  61:102744  PubMed ID: 35292424   DOI: 10.1016/j.scr.2022.102744

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