Cell No. : Cell Name
HPS4874 :
update : 2025/01/16
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| Comment | Disease specific iPS cell line derived from a patient : Dilated cardiomyopathy (dilated phase hypertrophic cardiomyopathy). Genome-edited iPS cell line. HPS4873, HPS4874, HPS4875, HPS4876 were derived from the same patient. |
| Comment from the depositor | Disease-specific iPS cells. iPS cells were established from the peripheral blood mononuclear cells of dilated cardiomyopathy (dilated phase hypertrophic cardiomyopathy) patient using Sendai virus vectors encoding OCT3/4, SOX2, KLF4, c-MYC. |
| Terms and conditions | 1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 3) The RECIPIENT agrees to use the BIOLOGICAL RESOURSE only for publication of research papers. 4) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 5) Contact Us : RIKEN Cell Bank |
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0065.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Year of deposit |
2020
|
| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Male
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| Age at sampling |
60s
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| Tissue |
peripheral blood mononuclear cell
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| Disease name |
Dilated cardiomyopathy (dilated phase hypertrophic cardiomyopathy), mutation in TNNT2 (c.478-480del, p.E160del)
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| Classification |
iPS
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| Recombinant |
recombinant
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| Genome Editing |
CRISPR/Cas9
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| Exogene |
OCT3/4, SOX2, KLF4, L-MYC
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| Vector |
Sendai virus vector, CytoTune®-iPS 2.0L
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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StemFit AK02N
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| Antibiotics |
|
Free
|
|
Passage method |
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TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
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| Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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Culture information
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Passage cell No |
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2.6-26x10 4 cells/60 mm dish
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| Temperature |
|
37
℃
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| CO2 concentration |
|
5
%
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| Feeder cells |
|
Free
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| Freeze medium |
|
STEM-CELLBANKER
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Virus (HIV) |
|
Undetected
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| Virus (HTLV-1) |
|
(-)
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| Others |
|
Use 10μM Y-27632 when thawing and passaging. Remove it next day.
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| STR(human) |
|
OK
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| Reference information |
Reference |
1
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| User's Publication |
0
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| Reference |
|
16788
Takumi Kondo, Shuichiro Higo, Mikio Shiba, Yasuaki Kohama, Satoshi Kameda, Tomoka Tabata, Hiroyuki Inoue, Shota Okuno, Shou Ogawa, Satoki Nakamura, Maki Takeda, Emiko Ito, Junjun Li, Li Liu, Yuki Kuramoto, Jong-Kook Lee, Seiji Takashima, Shigeru Miyagawa, Yoshiki Sawa, Shungo Hikoso, Yasushi Sakata
Human-Induced Pluripotent Stem Cell–Derived Cardiomyocyte Model for TNNT2 Δ160E-Induced Cardiomyopathy
Circulation: Genomic and Precision Medicine
2022
PubMed ID: 35861968
DOI: 10.1161/circgen.121.003522
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