Cell No. : Cell Name
HPS4292 : 414C2
update : 2024/04/17
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| Comment | Human iPS cell line derived from a healthy individual. HPS0002, HPS0063, HPS0076, HPS0296, HPS0326, HPS3668, HPS4290, HPS4291, HPS4292 were derived from the same person. Cryopreserved by vitrification method. |
| Comment from the depositor | Human iPS cell line. This line was established by introduction of episomal vectors encoding 6 factors (Oct3/4, Sox2, Klf4, MycL, Lin28 and shRNA for TP53) This transgenes were not detected in the established cells. |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Expected time |
|
| Year of deposit |
2019
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Caucasian
|
| Gender |
Female
|
| Age at sampling |
36
|
| Tissue |
skin
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, MycL, Lin28, shRNA for TP53
|
| Vector |
pCXLE
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_DP60
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
Primate ES Cell Medium + 4ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin
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Culture information
|
Passage ratio |
|
1 : 3-5 split
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
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| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.5-2.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma |
|
(-)
|
| Chromosome mode |
|
46,XX[20] at passage No. 16
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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