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Cell No. : Cell Name
HPS4290 : 201B7-Ff  update : 2024/05/29
CommentHuman iPS cell line derived from a healthy individual. HPS0002, HPS0063, HPS0076, HPS0296, HPS0326, HPS3668, HPS4290, HPS4291, HPS4292 were derived from the same person.
Comment from the depositorHuman iPS cell line. This line was established by introduction of retroviral vectors encoding 4 factors (Oct3/4, Sox2, Klf4, c-Myc). This line was on-feeder culture but moved to feeder-less.
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Expected time
Year of deposit 2019
Animal _human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 36
Tissue skin
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc
Vector MMLV retroviral vector (pMXs)
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freezing method Slow freezing
Mycoplasma (-)
Chromosome mode 46,XX[20]
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Reference information Reference 0
User's Publication 3

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User's Publication
19706  Bamba K, Ozawa M, Daitoku H, Kohara A.  Diverting the food-freezing technology improves the cryopreservation efficiency of induced pluripotent stem cells and derived neurospheres  Regen Ther  2024  27:83-91  PubMed ID: 38525239   DOI: 10.1016/j.reth.2024.03.007
21571  Nakamura T, Ueda J, Mizuno S, Honda K, Kazuno AA, Yamamoto H, Hara T, Takata A.  Topologically associating domains define the impact of de novo promoter variants on autism spectrum disorder risk.  Cell Genom  2024  100488  PubMed ID: 38280381   DOI: 10.1016/j.xgen.2024.100488
19863  Nakano-Kobayashi A, Canela A, Yoshihara T, Hagiwara M.  Astrocyte-targeting therapy rescues cognitive impairment caused by neuroinflammation via the Nrf2 pathway  Proc Natl Acad Sci U S A  2023  120(33):e2303809120  PubMed ID: 37549281   DOI: 10.1073/pnas.2303809120

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