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Cell No. : Cell Name
HPS4272 : update : 2024/11/18 |
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| Comment | Disease specific iPS cell line derived from a patient : Down syndrome, trisomy 21. HPS4270, HPS4271, HPS4272, HPS4273 were derived from the same patient. | |||
| Comment from the depositor | Induced disomy 21 iPS cell line [46,XY] by elimination of the maternal origin of the chromosome 21 (M1) from HPS4270 (Disease specific iPS cell line derived from a person with Down syndrome, [47,XY,+21]) | |||
| Terms and conditions | The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies) . | |||
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. | |||
| Order Form | Order Form(C-0042.pdf) MTA(C-0065.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Year of deposit | 2019 | ||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Gender | Male | |||
| Age at sampling | under 10 years of age | |||
| Tissue | skin | |||
| Disease name | Down syndrome | |||
| Classification | iPS | |||
| Recombinant | recombinant | |||
| Exogene | Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53-shRNA, EBNA1 | |||
| Vector | pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXWB-EBNA1 | Lifespan | infinite | |
| Morphology | ES-like | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | StemFit AK02N | |||
| Antibiotics | Free | Passage method | TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1 | |
| Coating dish | see Others (2) | Culture information | Passage cell No | 2.6-26x10 4 cells/60 mm dish |
| SC frequency | Subculture : once/5-8 days, Medium Renewal : every day or every other day | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Feeder cells | Free | |||
| Freeze medium | STEM-CELLBANKER | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Others | (1) Use 10μM Y-27632 when thawing and passaging. (2) Add and mix Laminin-511 E8 (iMatrix511) to the cell suspension at thawing (3.2 ug/mL) and passaging (0.4 ug/mL). | |||
| STR(human) | OK | |||
| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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