Cell No. : Cell Name HPS4272 :  update : 2024/11/18
Comment			Disease specific iPS cell line derived from a patient : Down syndrome, trisomy 21. HPS4270, HPS4271, HPS4272, HPS4273 were derived from the same patient.
Comment from the depositor			Induced disomy 21 iPS cell line [46,XY] by elimination of the maternal origin of the chromosome 21 (M1) from HPS4270 (Disease specific iPS cell line derived from a person with Down syndrome, [47,XY,+21])
Terms and conditions			The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies) .
Remarks			CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
Order Form			Order Form(C-0042.pdf)   MTA(C-0065.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Year of deposit	2019
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	under 10 years of age
		Tissue	skin
		Disease name	Down syndrome
		Classification	iPS
		Recombinant	recombinant
		Exogene	Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53-shRNA, EBNA1
		Vector	pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXWB-EBNA1
		Lifespan	infinite
		Morphology	ES-like
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		StemFit AK02N
		Antibiotics		Free
		Passage method		TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
		Coating dish		see Others (2)
	Culture information	Passage cell No		2.6-26x10 4 cells/60 mm dish
		SC frequency		Subculture : once/5-8 days, Medium Renewal : every day or every other day
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		Free
		Freeze medium		STEM-CELLBANKER
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Others		(1) Use 10μM Y-27632 when thawing and passaging. (2) Add and mix Laminin-511 E8 (iMatrix511) to the cell suspension at thawing (3.2 ug/mL) and passaging (0.4 ug/mL).
		STR(human)		OK
Reference information		Reference	1
		User's Publication	2

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Reference

16481  Wakita S, Hara M, Kitabatake Y, Kawatani K, Kurahashi H, Hashizume R.  Experimental method for haplotype phasing across the entire length of chromosome 21 in trisomy 21 cells using a chromosome elimination technique  J Hum Genet   2022  May 31  PubMed ID: 35637312   DOI: 10.1038/s10038-022-01049-6

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User's Publication

22425  Lam XJ, Maniam S, Ling KH, Cheah PS.  Lithium restores nuclear REST and Mitigates oxidative stress in down syndrome iPSC-Derived neurons.  Neuroscience  2025  567:86-95  PubMed ID: 39756608   DOI: 10.1016/j.neuroscience.2024.12.061

22477  Huang T, Fakurazi S, Cheah PS, Ling KH.  Dysregulation of REST and its target genes impacts the fate of neural progenitor cells in down syndrome.  Sci Rep  2025  15(1):2818  PubMed ID: 39843579   DOI: 10.1038/s41598-025-87314-y