Cell No. : Cell Name
HPS3854 :
update : 2025/03/24
|
| Comment | Disease specific iPS cell line derived from a patient : Alzheimer disease (Coriell Institute, AG07671). |
| Comment from the depositor | Alzheimer's disease, episomal vector (Oct3/4, p53-shRNA, Sox2, KLF4, L-MYC, LIN28) |
| Terms and conditions | |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Year of deposit |
2018
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
44
|
| Primary focus |
skin
|
| Disease name |
Alzheimer disease
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, p53-shRNA, Sox2, KLF4, L-MYC, LIN28
|
| Vector |
episomal vector
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
| Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix-511)
|
Culture information
|
Passage cell No |
|
2.6-26x10 4 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
STEM-CELLBANKER
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Others |
|
Use 10μM Y-27632 when thawing and passaging. Remove it next day.
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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