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Cell No. : Cell Name
HPS3844 :  update : 2023/07/26
CommentDisease specific iPS cell line derived from a patient : Fahr disease. HPS3844, HPS3845, HPS3846 were derived from the same patient.
Comment from the depositorFahr disease, episomal vector (Oct3/4, mp53DD, Sox2, Klf4, L-Myc, LIN28, EBNA1)
Terms and conditions1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies) . 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) Attach Appendix (non-for-profit) to MTA (C-0007) . 4) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Year of deposit 2017
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 70s
Tissue blood mononuclear cell
Disease name Fahr disease
Classification iPS
Recombinant recombinant
Exogene Oct3/4, mp53DD, Sox2, Klf4, L-Myc, LIN28, EBNA1
Vector episomal vector
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_A3LU
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium STEM-CELLBANKER
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Reference information Reference 0
User's Publication 0


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