Cell No. : Cell Name
HPS3386 :
update : 2025/01/16
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| Comment | Human iPS cell line derived from a healthy individual. HPS3386, HPS3387, HPS3388, HPS3389, HPS3390, HPS3391 were derived from the same person. |
| Comment from the depositor | Healthy donor iPS cells. iPS cells were established from the peripheral blood mononuclear cells of healthy donor using episomal vectors encoding OCT3/4, SOX2, KLF4, L-MYC, LIN28, mp53DD and EBNA1. |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . 3) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2016
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
50s
|
| Tissue |
peripheral blood mononuclear cell
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, mp53DD, EBNA1
|
| Vector |
pCE-hOCT3/4, pCE-hSK, pCE-hUL, pCE-mp53DD, pCXB-EBNA1
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_A3LG
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
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| Coating dish |
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0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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Culture information
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Passage cell No |
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2.6-26x10 4 cells/60 mm dish
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| SC frequency |
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Subculture : once/5-8 days, Medium Renewal : every day or every other day
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
STEM-CELLBANKER
|
| Mycoplasma/Acholeplasma |
|
(-)
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| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
2
|
| User's Publication |
|
22316
Mori H, Xu D, Shimoda Y, Yuan Z, Murakata Y, Xi B, Sato K, Yamamoto M, Tajiri K, Ishizu T, Ieda M, Murakoshi N.
Metabolic remodeling and calcium handling abnormality in induced pluripotent stem cell-derived cardiomyocytes in dilated phase of hypertrophic cardiomyopathy with MYBPC3 frameshift mutation
Sci Rep
2024
14(1):15422
PubMed ID: 38965264
DOI: 10.1038/s41598-024-62530-0
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20680
Shimoda Y, Murakoshi N, Mori H, Xu D, Tajiri K, Hemmi Y, Sato I, Noguchi M, Nakamura Y, Hayashi Y, Ieda M.
Generation of a human induced pluripotent stem cell line derived from a patient with dilated cardiomyopathy carrying LMNA nonsense mutation.
Stem Cell Res
2022
62:102793
PubMed ID: 35500377
DOI: 10.1016/j.scr.2022.102793
|
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HPS3386_HLA.jpg