Cell No. : Cell Name
HPS1887 :
update : 2023/06/14
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| Comment | Disease specific iPS cell line derived from a patient : Spinocerebellar degeneration (SCA6). Analysis of causal genetic changes.Cryopreserved by vitrification method. |
| Comment from the depositor | Disease specific iPS cell line derived from a patient : Spinocerebellar degeneration (SCA6). |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Rep. 2016;17:1482-1490) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 5) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2016
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
60s
|
| Tissue |
skin
|
| Disease name |
Spinocerebellar degeneration (SCA6)
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4-shp53, Sox2, Klf4, L-Myc, Lin28
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM/HamF12 (2.5mM GlutaMAX) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
|
0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
|
| SC frequency |
|
Subculture : once/4-6 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
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| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.5-2.5 x 10 6 cells/100 mm dish
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| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
|
Vitrification
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| Mycoplasma/Acholeplasma |
|
(-)
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| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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