Cell No. : Cell Name HPS1745 : CiRA00003  update : 2023/06/07
Comment			Disease specific iPS cell line derived from a patient : Alzheimer disease. HPS1747 was derived from the same patient. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions			1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies) . 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Stem Cell 2013;12:487-496) designated by the DEPOSITOR is required. 4) Contact Us : RIKEN Cell Bank
Remarks
Order Form			Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	https://www.cira.kyoto-u.ac.jp/
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	50s
		Tissue	skin
		Disease name	alzheimer disease
		Classification	iPS
		Recombinant	recombinant
		Exogene	Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53, EBNA1
		Lifespan	infinite
		Morphology	ES-like
		Cellosaurus(Expasy)	CVCL_UP55
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		Primate ES Cell Medium + 4ng/ml human basic FGF
		Antibiotics		Free
		Passage method		0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage ratio		1 : 1.5-3 split
		SC frequency		Subculture : once/4-6 days, Medium Renewal : every day
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		SNL 76/7
		Feeder treatment		X-rays 5000R (or MMC)
		Feeder seed cells		1.5-2.5 x 10 6 cells/100 mm dish
		Freeze medium		DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
		Freezing method		Vitrification
		Mycoplasma		(-)
		STR(human)		OK
Images			deposit info	lot info
Relational File			deposit info	lot info
				SDS_DAP(MEM)_Japanese.pdf
Reference information		Reference	2
		User's Publication	1

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Reference

4005  Kondo T, Imamura K, Funayama M, Tsukita K, Miyake M, Ohta A, Woltjen K, Nakagawa M, Asada T, Arai T, Kawakatsu S, Izumi Y, Kaji R, Iwata N, Inoue H.  iPSC-Based Compound Screening and In Vitro Trials Identify a Synergistic Anti-amyloid β Combination for Alzheimer's Disease.  Cell Rep  2017  21:2304-2312  PubMed ID: 29166618   DOI: 10.1016/j.celrep.2017.10.109

4004  Kondo T, Asai M, Tsukita K, Kutoku Y, Ohsawa Y, Sunada Y, Imamura K, Egawa N, Yahata N, Okita K, Takahashi K, Asaka I, Aoi T, Watanabe A, Watanabe K, Kadoya C, Nakano R, Watanabe D, Maruyama K, Hori O, Hibino S, Choshi T, Nakahata T, Hioki H, Kaneko T, Naitoh M, Yoshikawa K, Yamawaki S, Suzuki S, Hata R, Ueno S, Seki T, Kobayashi K, Toda T, Murakami K, Irie K, Klein WL, Mori H, Asada T, Takahashi R, Iwata N, Yamanaka S, Inoue H.  Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular Aβ and differential drug responsiveness.  Cell Stem Cell  2013  12:487-496  PubMed ID: 23434393   DOI: 10.1016/j.stem.2013.01.009

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User's Publication

17483  Nakatsu D, Kunishige R, Taguchi Y, Shinozaki-Narikawa N, Osaka K, Yokomizo K, Ishida M, Takei S, Yamasaki S, Hagiya K, Hattori K, Tsukamoto T, Murata M, Kano F.  BMP4-SMAD1/5/9-RUNX2 pathway activation inhibits neurogenesis and oligodendrogenesis in Alzheimer's patients' iPSCs in senescence-related conditions  Stem Cell Reports  2023  18(3):688-705  PubMed ID: 36764297   DOI: 10.1016/j.stemcr.2023.01.004