Cell No. : Cell Name
HPS1655 :
update : 2023/10/16
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| Comment | Disease specific iPS cell line derived from a patient : PCDH19 epilepsy, PCDH19 mutation. HPS1655, HPS1656, HPS1657, HPS1658, HPS1659, HPS1660 were derived from the same patient. |
| Comment from the depositor | Disease-specific iPS cells. iPS cells were established from the peripheral blood mononuclear cells of PCDH19 epilepsy disease patient using episomal vectors encoding OCT3/4, SOX2, KLF4, L-MYC, LIN28, mp53DD and EBNA1. |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . 3) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2015
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
10s
|
| Tissue |
peripheral blood mononuclear cell
|
| Disease name |
PCDH19 epilepsy, PCDH19 mutation
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, mp53DD, EBNA1
|
| Vector |
pCE-hOCT3/4, pCE-hSK, pCE-hUL, pCE-mp53DD, pCXB-EBNA1
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_C9RV
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
| Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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Culture information
|
Passage cell No |
|
2.6-26x10 4 cells/60 mm dish
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| SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
StemFit AK02N + 10 μM Y-27632 + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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