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Cell No. : Cell Name
HPS1629 :  update : 2021/12/22
CommentDisease specific iPS cell line derived from a patient : 22q11.2 deletion syndrome. HPS1627, HPS1628, HPS1629, HPS1630, HPS1631 were derived from the same patient.
Comment from the depositorDisease-specific iPS cells. iPS cells were established from the peripheral blood mononuclear cells of 22q11.2 deletion syndrome patient using episomal vectors encoding OCT3/4, SOX2, KLF4, L-MYC, LIN28, mp53DD and EBNA1.
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach TERMS and CONDITIONS (non-for-profit) or TERMS and CONDITIONS (for-profit) to MTA (C-0007 or C-0007p) . 3) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Year of deposit 2015
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling under 10 years of age
Tissue peripheral blood mononuclear cell
Disease name 22q11.2 deletion syndrome
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, mp53DD, EBNA1
Vector pCE-hOCT3/4, pCE-hSK, pCE-hUL, pCE-mp53DD, pCXB-EBNA1
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_YS38
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm^(2) Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10^(4) cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium STEM-CELLBANKER
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Images
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lot info
Reference information Reference 0
User's Publication 0


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