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Cell No. : Cell Name
HPS1627 :  update : 2024/05/14
CommentDisease specific iPS cell line derived from a patient : 22q11.2 deletion syndrome. The analysis performed by RIKEN BRC indicated hemizygous deletion of 18,917,031_21,798,907 in 22q11.21 region (detected by CGH array). HPS1627, HPS1628, HPS1629, HPS1630, HPS1631 were derived from the same patient.
Comment from the depositorDisease-specific iPS cells. iPS cells were established from the peripheral blood mononuclear cells of 22q11.2 deletion syndrome patient using episomal vectors encoding OCT3/4, SOX2, KLF4, L-MYC, LIN28, mp53DD and EBNA1.
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . 3) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Year of deposit 2015
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling under 10 years of age
Tissue peripheral blood mononuclear cell
Disease name 22q11.2 deletion syndrome
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, mp53DD, EBNA1
Vector pCE-hOCT3/4, pCE-hSK, pCE-hUL, pCE-mp53DD, pCXB-EBNA1
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_YS36
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium StemFit AK02N + 10 μM Y-27632 + 10% DMSO
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
20618  Shimizu T, Matsuo-Takasaki M, Luijkx D, Takami M, Arai Y, Noguchi M, Nakamura Y, Hayata T, Saito MK, Hayashi Y.  Generation of human induced pluripotent stem cell lines derived from four DiGeorge syndrome patients with 22q11.2 deletion.  Stem Cell Res  2022  61:102744  PubMed ID: 35292424   DOI: 10.1016/j.scr.2022.102744



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