Search
CELL BANK Website

Back Back
Cell No. : Cell Name
HPS1134 :  update : 2024/05/07
CommentDisease specific iPS cell line derived from a patient : Diamond-Blackfan anemia. HPS1126, HPS1127, HPS1128, HPS1132 and HPS1133 were derived from the same patient.
Comment from the depositor
Terms and conditions1) The RECIPIENT Scientist belongs to a not-for-profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) Attach Appendix (non-for-profit) to MTA (C-0007). 3) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Year of deposit 2015
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 10s
Tissue skin
Disease name Diamond-Blackfan anemia
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53, EBNA1
Vector pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXWB-EBNA1
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_UN25
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium StemFit AK02N + 10 μM Y-27632 + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 0


To topTop
Reference

To topTop
User's Publication



Back Back Return Top Page