Cell No. : Cell Name
HPS1133 :
update : 2024/05/07
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| Comment | Disease specific iPS cell line derived from a patient : Diamond-Blackfan anemia. HPS1126, HPS1127, HPS1128, HPS1132 and HPS1134 were derived from the same patient. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT Scientist belongs to a not-for-profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) Attach Appendix (non-for-profit) to MTA (C-0007). 3) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2015
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
10s
|
| Tissue |
skin
|
| Disease name |
Diamond-Blackfan anemia
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53, EBNA1
|
| Vector |
pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXWB-EBNA1
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_UN24
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
| Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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Culture information
|
Passage cell No |
|
2.6-26x10 4 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
StemFit AK02N + 10 μM Y-27632 + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
0
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