Cell No. : Cell Name
HPS1046 :
update : 2025/01/16
|
Comment | Disease specific iPS cell line derived from a healthy person. |
Comment from the depositor | |
Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . 3) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 4) Contact Us : RIKEN Cell Bank |
Remarks | |
Order Form |
Order Form(C-0042.pdf)
 
Approval Form(C-0057.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
Basic information
|
Year of deposit |
2015
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Race |
Japanese
|
Gender |
Male
|
Age at sampling |
40s
|
Tissue |
blood mononuclear cell
|
Classification |
iPS
|
Recombinant |
recombinant
|
Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, mp53DD, EBNA1
|
|
Lifespan |
infinite
|
Morphology |
ES-like
|
Cellosaurus(Expasy) |
CVCL_UN19
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
StemFit AK02N
|
Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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Culture information
|
Passage cell No |
|
2.6-26x10 4 cells/60 mm dish
|
SC frequency |
|
Subculture : once/5-10 days, Medium Renewal : every day or every other day
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Feeder cells |
|
Free
|
Freeze medium |
|
StemFit AK02N + 10 μM Y-27632 + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma/Acholeplasma |
|
(-)
|
Virus (HIV) |
|
Undetected
|
Virus (HTLV-1) |
|
(-)
|
Chromosome mode |
|
46,XY [20]
|
Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
STR(human) |
|
OK
|
Images |
deposit info | lot info |
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|
Reference information |
Reference |
2
|
User's Publication |
2
|
Reference |
4005
Kondo T, Imamura K, Funayama M, Tsukita K, Miyake M, Ohta A, Woltjen K, Nakagawa M, Asada T, Arai T, Kawakatsu S, Izumi Y, Kaji R, Iwata N, Inoue H.
iPSC-Based Compound Screening and In Vitro Trials Identify a Synergistic Anti-amyloid β Combination for Alzheimer's Disease.
Cell Rep
2017
21:2304-2312
PubMed ID: 29166618
DOI: 10.1016/j.celrep.2017.10.109
|
4543
Matsuzono K, Imamura K, Murakami N, Tsukita K, Yamamoto T, Izumi Y4, Kaji R, Ohta Y, Yamashita T, Abe K, Inoue H.
Antisense Oligonucleotides Reduce RNA Foci in Spinocerebellar Ataxia 36 Patient iPSCs.
Mol Ther Nucleic Acids
2017
15;8:211-219
PubMed ID: 28918022
DOI: 10.1016/j.omtn.2017.06.017
|
User's Publication |
19551
Nonaka H, Kondo T, Suga M, Yamanaka R, Sagara Y, Tsukita K, Mitsutomi N, Homma K, Saito R, Miyoshi F, Ohzeki H, Okuyama M, Inoue H.
Induced pluripotent stem cell-based assays recapture multiple properties of human astrocytes
J Cell Mol Med
2024
28(7):e18214
PubMed ID: 38509731
DOI: 10.1111/jcmm.18214
|
11410
Sekine SI, Nishii K, Masaka T, Kurita H, Inden M, Hozumi I.
SLC20A2 variants cause dysfunctional phosphate transport activity in endothelial cells induced from Idiopathic Basal Ganglia Calcification patients-derived iPSCs.
Biochem. Biophys. Res. Commun.
2019
PubMed ID: 30704756
DOI: 10.1016/j.bbrc.2019.01.096
|