Cell No. : Cell Name
HPS1011 :
update : 2023/06/07
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| Comment | Disease specific iPS cell line derived from a patient : Familial central diabetes insipidus. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 4) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
30s
|
| Tissue |
peripheral blood mononuclear cells
|
| Disease name |
Familial central diabetes insipidus
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, mp53DD, EBNA1
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_VM97
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| |
deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM/HamF12 (2.5mM GlutaMAX) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 2-3 split
|
| SC frequency |
|
Subculture : once/4-6 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
| Reference |
|
20899
Hajime Ozaki, Hidetaka Suga, Mayu Sakakibara, Mika Soen, Natsuki Miyake, Tsutomu Miwata, Shiori Taga, Takashi Nagai, Mayuko Kano, Kazuki Mitsumoto, Takashi Miyata, Tomoko Kobayashi, Mariko Sugiyama, Takeshi Onoue, Hiroshi Takagi, Daisuke Hagiwara, Shintaro Iwama, Ryoichi Banno, Genzo Iguchi, Yutaka Takahashi, Keiko Muguruma, Haruhisa Inoue & Hiroshi Arima
Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive state
Scientific Reports
2022
17381
PubMed ID: 36253431
DOI: 10.1038/s41598-022-22405-8
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