細胞番号 : 細胞名
HPS1006 : 1383D6
update : 2024/12/05
|
| 細胞特性(Comment:英) | Human iPS cell line derived from a healthy individual. |
| 細胞特性(日) | ヒト人工多能性幹(iPS)細胞株。Feeder free培養。緩慢冷却法保存。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . |
| 使用条件(日) | 1) 非営利機関以外の利用者は事前にiPSアカデミアジャパン株式会社 から確認書を得ること。2) 別紙1 (非営利) または別紙1 (営利) を提供同意書 (C-0003またはC-0003p) に添付すること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0041.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Yamanaka, Shinya
|
| 寄託日 |
2015
|
| 動物種 |
_human
< Mammals
|
| 属名 |
Homo
|
| 種名 |
sapiens
|
| 人種 |
Asian
|
| 性別 |
Male
|
| 齢 |
36
|
| 採取組織 |
peripheral blood
|
| 細胞分類 |
iPS
|
| 遺伝子改変 |
recombinant
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_UP39
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Adherent cells
|
| 培地 |
|
StemFit AK02N
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
| 培養容器のコーティング |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
|
|
播種細胞数 |
|
2.6-26x10 4 cells/60 mm dish
|
| 継代・培地交換頻度 |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| フィーダー細胞 |
|
Free
|
| 凍結培地 |
|
STEM-CELLBANKER
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ/アコレプラズマ |
|
(-)
|
| ウィルス（HIV) |
|
Undetected
|
| ウィルス（HTLV-1) |
|
(-)
|
| 染色体検査 |
|
46,XY [20]
|
| その他 |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
| 個体識別検査 |
|
OK
|
| 画像情報 |
寄託時情報 | ロット情報 |
|
|
|
| 文献情報 |
Reference(英) |
3件
|
| Reference(日) |
0件
|
| 利用者成果(英) |
15件
|
| 利用者成果(日) |
0件
|
| Reference(英) |
|
17745
Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H
Programmable mammalian translational modulators by CRISPR-associated proteins
Nat Commun
2023
14(1):2243
PubMed ID: 37076490
DOI: 10.1038/s41467-023-37540-7
|
|
20937
Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.
In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.
PLoS One
2022
17(11)6:e0275600
PubMed ID: 36378656
DOI: 10.1371/journal.pone.0275600
|
|
4002
Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.
Sci Rep
2014
4:3594
PubMed ID: 24399248
DOI: 10.1038/srep03594
|
| 利用者成果(英) |
|
21921
Kinya Matsuo, Jun Nagamatsu, Kazuhiro Nagata, Ryusei Umeda, Takaya Shiota, Satoru Morimoto, Naoki Suzuki, Masashi Aoki, Hideyuki Okano, Masayuki Nakamori, Hideaki Nishihara
Establishment of a novel amyotrophic lateral sclerosis patient (TARDBPN345K/+)-derived brain microvascular endothelial cell model reveals defective Wnt/β-catenin signaling: investigating diffusion barrier dysfunction and immune cell interaction
Frontiers in Cell and Developmental Biology
2024
12
DOI: 10.3389/fcell.2024.1357204
|
|
21986
Yohei Hayashi, Koji Nakade
Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing
Neuromethods
2024
85-92
DOI: 10.1007/978-1-0716-3999-3_7
|
|
22113
Li R, Tsuboi H, Ito H, Takagi D, Chang YH, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Ohnuma K, Takahashi S, Hayashi Y.
Generation of human induced pluripotent stem cell lines derived from two glucose transporter 1 deficiency syndrome patients.
Stem Cell Res
2024
81:103584
PubMed ID: 39490212
DOI: 10.1016/j.scr.2024.103584
|
|
22139
Ge N, Suzuki K, Sato I, Noguchi M, Nakamura Y, Matsuo-Takasaki M, Fujishiro J, Hayashi Y.
Generation of human induced pluripotent stem cell lines derived from patients of cystic biliary atresia.
Hum Cell
2024
38(1):18
PubMed ID: 39532815
DOI: 10.1007/s13577-024-01147-x
|
|
22149
Takagi D, Tsukamoto S, Nakade K, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Yumoto N, Kawada J, Hayata T, Hayashi Y.
Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization.
Stem Cell Res
2024
79:103493
PubMed ID: 39032428
DOI: 10.1016/j.scr.2024.103493
|
|
22171
Matsuo-Takasaki M, Kambayashi S, Hemmi Y, Wakabayashi T, Shimizu T, An Y, Ito H, Takeuchi K, Ibuki M, Kawashima T, Masayasu R, Suzuki M, Kawai Y, Umekage M, Kato TM, Noguchi M, Nakade K, Nakamura Y, Nakaishi T, Nishishita N, Tsukahara M, Hayashi Y.
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.
Elife
2024
12
PubMed ID: 39529479
DOI: 10.7554/eLife.89724
|
|
21262
Matsuo K, Engelhardt B, Nishihara H.
Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype.
J Vis Exp
2023
(195)
PubMed ID: 37318257
DOI: 10.3791/65134
|
|
21311
Kaneko H, Kaitsuka T, Tomizawa K.
Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.
Cell Mol Life Sci
2023
80(8):200
PubMed ID: 37421441
DOI: 10.1007/s00018-023-04847-z
|
|
21322
Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.
Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.
Sci Rep
2023
13(1):11610
PubMed ID: 37463955
DOI: 10.1038/s41598-023-38548-1
|
|
21492
Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.
Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
Cell Rep
2023
113431
PubMed ID: 38039961
DOI: 10.1016/j.celrep.2023.113431
|
|
21497
Koji Nakade, Satomi Tsukamoto, Kenichi Nakashima, Yuri An, Iori Sato, Jingyue Li, Yuzuno Shimoda, Yasuko Hemmi, Yoshihiro Miwa, Yohei Hayashi
Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system
Cell Reports Methods
2023
PubMed ID: 38086384
DOI: 10.1016/j.crmeth.2023.100662
|
|
20735
Ura H, Togi S, Niida Y.
Poly(A) capture full length cDNA sequencing improves the accuracy and detection ability of transcript quantification and alternative splicing events.
Sci Rep
2022
12(1):10599
PubMed ID: 35732903
DOI: 10.1038/s41598-022-14902-7
|
|
20868
Takamatsu G, Manome Y, Lee JS, Toyama K, Hayakawa T, Hara-Miyauchi C, Hasegawa-Ogawa M, Katagiri C, Kondo T, James Okano H, Matsushita M.
Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling.
Stem Cell Res
2022
64:102915
PubMed ID: 36166871
DOI: 10.1016/j.scr.2022.102915
|
|
20174
Tsukamoto S, Nakade K, Wakabayashi T, Nakashima K, Takami M, Hemmi Y, Kuramochi Y, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Miwa Y, Hayashi Y.
Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing.
Stem Cell Res
2021
53:102363
PubMed ID: 34087992
DOI: 10.1016/j.scr.2021.102363
|
|
13064
Maurissen TL, Woltjen K.
Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation.
Nat Commun
2020
PubMed ID: 32513994
DOI: 10.1038/s41467-020-16643-5
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト