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Cell No. : Cell Name
HPS1006 : 1383D6  update : 2023/10/12
CommentHuman iPS cell line derived from healthy individual.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamanaka, Shinya
Year of deposit 2015
Animal _human < Mammals
Genus Homo
Species sapiens
Race Asian
Gender Male
Age at sampling 36
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_UP39
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium StemFit AK02N + 10 μM Y-27632 + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XY [20]
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 6


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Reference
17745  Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H  Programmable mammalian translational modulators by CRISPR-associated proteins  Nat Commun  2023  14(1):2243  PubMed ID: 37076490   DOI: 10.1038/s41467-023-37540-7
20937  Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.  In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.  PLoS One  2022  17(11)6:e0275600  PubMed ID: 36378656   DOI: 10.1371/journal.pone.0275600
4002  Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.  Sci Rep  2014  4:3594  PubMed ID: 24399248   DOI: 10.1038/srep03594

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User's Publication
21262  Matsuo K, Engelhardt B, Nishihara H.  Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype.  J Vis Exp  2023  (195)  PubMed ID: 37318257   DOI: 10.3791/65134
21311  Kaneko H, Kaitsuka T, Tomizawa K.  Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.  Cell Mol Life Sci  2023  80(8):200  PubMed ID: 37421441   DOI: 10.1007/s00018-023-04847-z
21322  Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.  Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.  Sci Rep  2023  13(1):11610  PubMed ID: 37463955   DOI: 10.1038/s41598-023-38548-1
20735  Ura H, Togi S, Niida Y.  Poly(A) capture full length cDNA sequencing improves the accuracy and detection ability of transcript quantification and alternative splicing events.  Sci Rep  2022  12(1):10599  PubMed ID: 35732903   DOI: 10.1038/s41598-022-14902-7
20868  Takamatsu G, Manome Y, Lee JS, Toyama K, Hayakawa T, Hara-Miyauchi C, Hasegawa-Ogawa M, Katagiri C, Kondo T, James Okano H, Matsushita M.  Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling.  Stem Cell Res  2022  64:102915  PubMed ID: 36166871   DOI: 10.1016/j.scr.2022.102915
20174  Tsukamoto S, Nakade K, Wakabayashi T, Nakashima K, Takami M, Hemmi Y, Kuramochi Y, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Miwa Y, Hayashi Y.  Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing.  Stem Cell Res  2021  53:102363  PubMed ID: 34087992   DOI: 10.1016/j.scr.2021.102363



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