Cell No. : Cell Name
HPS1006 : 1383D6
update : 2024/12/05
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Comment | Human iPS cell line derived from a healthy individual. |
Comment from the depositor | |
Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . |
Remarks | |
Order Form |
Order Form(C-0042.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Yamanaka, Shinya
|
Year of deposit |
2015
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Race |
Asian
|
Gender |
Male
|
Age at sampling |
36
|
Tissue |
peripheral blood
|
Classification |
iPS
|
Recombinant |
recombinant
|
|
Lifespan |
infinite
|
Morphology |
ES-like
|
Cellosaurus(Expasy) |
CVCL_UP39
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
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Adherent cells
|
Culture medium |
|
StemFit AK02N
|
Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
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Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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Culture information
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Passage cell No |
|
2.6-26x10 4 cells/60 mm dish
|
SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Feeder cells |
|
Free
|
Freeze medium |
|
STEM-CELLBANKER
|
Freezing method |
|
Slow freezing
|
Mycoplasma/Acholeplasma |
|
(-)
|
Virus (HIV) |
|
Undetected
|
Virus (HTLV-1) |
|
(-)
|
Chromosome mode |
|
46,XY [20]
|
Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
STR(human) |
|
OK
|
Images |
deposit info | lot info |
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|
Reference information |
Reference |
3
|
User's Publication |
15
|
Reference |
17745
Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H
Programmable mammalian translational modulators by CRISPR-associated proteins
Nat Commun
2023
14(1):2243
PubMed ID: 37076490
DOI: 10.1038/s41467-023-37540-7
|
20937
Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.
In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.
PLoS One
2022
17(11)6:e0275600
PubMed ID: 36378656
DOI: 10.1371/journal.pone.0275600
|
4002
Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.
Sci Rep
2014
4:3594
PubMed ID: 24399248
DOI: 10.1038/srep03594
|
User's Publication |
21921
Kinya Matsuo, Jun Nagamatsu, Kazuhiro Nagata, Ryusei Umeda, Takaya Shiota, Satoru Morimoto, Naoki Suzuki, Masashi Aoki, Hideyuki Okano, Masayuki Nakamori, Hideaki Nishihara
Establishment of a novel amyotrophic lateral sclerosis patient (TARDBPN345K/+)-derived brain microvascular endothelial cell model reveals defective Wnt/β-catenin signaling: investigating diffusion barrier dysfunction and immune cell interaction
Frontiers in Cell and Developmental Biology
2024
12
DOI: 10.3389/fcell.2024.1357204
|
21986
Yohei Hayashi, Koji Nakade
Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing
Neuromethods
2024
85-92
DOI: 10.1007/978-1-0716-3999-3_7
|
22113
Li R, Tsuboi H, Ito H, Takagi D, Chang YH, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Ohnuma K, Takahashi S, Hayashi Y.
Generation of human induced pluripotent stem cell lines derived from two glucose transporter 1 deficiency syndrome patients.
Stem Cell Res
2024
81:103584
PubMed ID: 39490212
DOI: 10.1016/j.scr.2024.103584
|
22139
Ge N, Suzuki K, Sato I, Noguchi M, Nakamura Y, Matsuo-Takasaki M, Fujishiro J, Hayashi Y.
Generation of human induced pluripotent stem cell lines derived from patients of cystic biliary atresia.
Hum Cell
2024
38(1):18
PubMed ID: 39532815
DOI: 10.1007/s13577-024-01147-x
|
22149
Takagi D, Tsukamoto S, Nakade K, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Yumoto N, Kawada J, Hayata T, Hayashi Y.
Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization.
Stem Cell Res
2024
79:103493
PubMed ID: 39032428
DOI: 10.1016/j.scr.2024.103493
|
22171
Matsuo-Takasaki M, Kambayashi S, Hemmi Y, Wakabayashi T, Shimizu T, An Y, Ito H, Takeuchi K, Ibuki M, Kawashima T, Masayasu R, Suzuki M, Kawai Y, Umekage M, Kato TM, Noguchi M, Nakade K, Nakamura Y, Nakaishi T, Nishishita N, Tsukahara M, Hayashi Y.
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.
Elife
2024
12
PubMed ID: 39529479
DOI: 10.7554/eLife.89724
|
21262
Matsuo K, Engelhardt B, Nishihara H.
Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype.
J Vis Exp
2023
(195)
PubMed ID: 37318257
DOI: 10.3791/65134
|
21311
Kaneko H, Kaitsuka T, Tomizawa K.
Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.
Cell Mol Life Sci
2023
80(8):200
PubMed ID: 37421441
DOI: 10.1007/s00018-023-04847-z
|
21322
Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.
Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.
Sci Rep
2023
13(1):11610
PubMed ID: 37463955
DOI: 10.1038/s41598-023-38548-1
|
21492
Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.
Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
Cell Rep
2023
113431
PubMed ID: 38039961
DOI: 10.1016/j.celrep.2023.113431
|
21497
Koji Nakade, Satomi Tsukamoto, Kenichi Nakashima, Yuri An, Iori Sato, Jingyue Li, Yuzuno Shimoda, Yasuko Hemmi, Yoshihiro Miwa, Yohei Hayashi
Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system
Cell Reports Methods
2023
PubMed ID: 38086384
DOI: 10.1016/j.crmeth.2023.100662
|
20735
Ura H, Togi S, Niida Y.
Poly(A) capture full length cDNA sequencing improves the accuracy and detection ability of transcript quantification and alternative splicing events.
Sci Rep
2022
12(1):10599
PubMed ID: 35732903
DOI: 10.1038/s41598-022-14902-7
|
20868
Takamatsu G, Manome Y, Lee JS, Toyama K, Hayakawa T, Hara-Miyauchi C, Hasegawa-Ogawa M, Katagiri C, Kondo T, James Okano H, Matsushita M.
Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling.
Stem Cell Res
2022
64:102915
PubMed ID: 36166871
DOI: 10.1016/j.scr.2022.102915
|
20174
Tsukamoto S, Nakade K, Wakabayashi T, Nakashima K, Takami M, Hemmi Y, Kuramochi Y, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Miwa Y, Hayashi Y.
Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing.
Stem Cell Res
2021
53:102363
PubMed ID: 34087992
DOI: 10.1016/j.scr.2021.102363
|
13064
Maurissen TL, Woltjen K.
Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation.
Nat Commun
2020
PubMed ID: 32513994
DOI: 10.1038/s41467-020-16643-5
|