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Cell No. : Cell Name
HPS1006 : 1383D6  update : 2025/03/26
CommentHuman iPS cell line derived from a healthy individual.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamanaka, Shinya
Year of deposit 2015
Animal _human < Mammals
Genus Homo
Species sapiens
Race Asian
Gender Male
Age at sampling 36
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_UP39
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium STEM-CELLBANKER
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XY [20]
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 16


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Reference
17745  Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H  Programmable mammalian translational modulators by CRISPR-associated proteins  Nat Commun  2023  14(1):2243  PubMed ID: 37076490   DOI: 10.1038/s41467-023-37540-7
20937  Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.  In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.  PLoS One  2022  17(11)6:e0275600  PubMed ID: 36378656   DOI: 10.1371/journal.pone.0275600
4002  Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.  Sci Rep  2014  4:3594  PubMed ID: 24399248   DOI: 10.1038/srep03594

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User's Publication
22414  Takeuchi K, Tabe S, Yamamoto Y, Takahashi K, Matsuo M, Ueno Y, Ohtsuka M, Morinaga S, Miyagi Y, Yamaguchi T, Tanimizu N, Taniguchi H.  Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment.  STAR Protoc  2025  6(1):103539  PubMed ID: 39772386   DOI: 10.1016/j.xpro.2024.103539
21921  Kinya Matsuo, Jun Nagamatsu, Kazuhiro Nagata, Ryusei Umeda, Takaya Shiota, Satoru Morimoto, Naoki Suzuki, Masashi Aoki, Hideyuki Okano, Masayuki Nakamori, Hideaki Nishihara  Establishment of a novel amyotrophic lateral sclerosis patient (TARDBPN345K/+)-derived brain microvascular endothelial cell model reveals defective Wnt/β-catenin signaling: investigating diffusion barrier dysfunction and immune cell interaction  Frontiers in Cell and Developmental Biology  2024  12    DOI: 10.3389/fcell.2024.1357204
21986  Yohei Hayashi, Koji Nakade  Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing  Neuromethods  2024  85-92    DOI: 10.1007/978-1-0716-3999-3_7
22113  Li R, Tsuboi H, Ito H, Takagi D, Chang YH, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Ohnuma K, Takahashi S, Hayashi Y.  Generation of human induced pluripotent stem cell lines derived from two glucose transporter 1 deficiency syndrome patients.  Stem Cell Res  2024  81:103584  PubMed ID: 39490212   DOI: 10.1016/j.scr.2024.103584
22139  Ge N, Suzuki K, Sato I, Noguchi M, Nakamura Y, Matsuo-Takasaki M, Fujishiro J, Hayashi Y.  Generation of human induced pluripotent stem cell lines derived from patients of cystic biliary atresia.  Hum Cell  2024  38(1):18  PubMed ID: 39532815   DOI: 10.1007/s13577-024-01147-x
22149  Takagi D, Tsukamoto S, Nakade K, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Yumoto N, Kawada J, Hayata T, Hayashi Y.  Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization.  Stem Cell Res  2024  79:103493  PubMed ID: 39032428   DOI: 10.1016/j.scr.2024.103493
22171  Matsuo-Takasaki M, Kambayashi S, Hemmi Y, Wakabayashi T, Shimizu T, An Y, Ito H, Takeuchi K, Ibuki M, Kawashima T, Masayasu R, Suzuki M, Kawai Y, Umekage M, Kato TM, Noguchi M, Nakade K, Nakamura Y, Nakaishi T, Nishishita N, Tsukahara M, Hayashi Y.  Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.  Elife  2024  12  PubMed ID: 39529479   DOI: 10.7554/eLife.89724
21262  Matsuo K, Engelhardt B, Nishihara H.  Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype.  J Vis Exp  2023  (195)  PubMed ID: 37318257   DOI: 10.3791/65134
21311  Kaneko H, Kaitsuka T, Tomizawa K.  Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.  Cell Mol Life Sci  2023  80(8):200  PubMed ID: 37421441   DOI: 10.1007/s00018-023-04847-z
21322  Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.  Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.  Sci Rep  2023  13(1):11610  PubMed ID: 37463955   DOI: 10.1038/s41598-023-38548-1
21492  Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.  Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.  Cell Rep  2023  113431  PubMed ID: 38039961   DOI: 10.1016/j.celrep.2023.113431
21497  Koji Nakade, Satomi Tsukamoto, Kenichi Nakashima, Yuri An, Iori Sato, Jingyue Li, Yuzuno Shimoda, Yasuko Hemmi, Yoshihiro Miwa, Yohei Hayashi  Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system  Cell Reports Methods  2023    PubMed ID: 38086384   DOI: 10.1016/j.crmeth.2023.100662
20735  Ura H, Togi S, Niida Y.  Poly(A) capture full length cDNA sequencing improves the accuracy and detection ability of transcript quantification and alternative splicing events.  Sci Rep  2022  12(1):10599  PubMed ID: 35732903   DOI: 10.1038/s41598-022-14902-7
20868  Takamatsu G, Manome Y, Lee JS, Toyama K, Hayakawa T, Hara-Miyauchi C, Hasegawa-Ogawa M, Katagiri C, Kondo T, James Okano H, Matsushita M.  Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling.  Stem Cell Res  2022  64:102915  PubMed ID: 36166871   DOI: 10.1016/j.scr.2022.102915
20174  Tsukamoto S, Nakade K, Wakabayashi T, Nakashima K, Takami M, Hemmi Y, Kuramochi Y, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Miwa Y, Hayashi Y.  Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing.  Stem Cell Res  2021  53:102363  PubMed ID: 34087992   DOI: 10.1016/j.scr.2021.102363
13064  Maurissen TL, Woltjen K.  Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation.  Nat Commun  2020    PubMed ID: 32513994   DOI: 10.1038/s41467-020-16643-5


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