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Cell No. : Cell Name
HPS1005 : 1383D2  update : 2024/05/29
CommentHuman iPS cell line derived from a healthy individual.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamanaka, Shinya
Year of deposit 2015
Animal _human < Mammals
Genus Homo
Species sapiens
Race Asian
Gender Male
Age at sampling 36
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_UP38
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium StemFit AK02N + 10 μM Y-27632 + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XY[19], 46,XY,add(4)(q35)[1]
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 4

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4002  Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.  Sci Rep  2014  4:3594  PubMed ID: 24399248   DOI: 10.1038/srep03594

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User's Publication
19819  Zhang D, Eguchi N, Okazaki S, Sora I, Hishimoto A.  Telencephalon Organoids Derived from an Individual with ADHD Show Altered Neurodevelopment of Early Cortical Layer Structure  Stem Cell Rev Rep  2023  19(5):1482-1491  PubMed ID: 36872412   DOI: 10.1007/s12015-023-10519-z
21492  Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.  Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.  Cell Rep  2023  113431  PubMed ID: 38039961   DOI: 10.1016/j.celrep.2023.113431
13374  Chang PH, Chao HM, Chern E, Hsu SH.  Chitosan 3D cell culture system promotes naïve-like features of human induced pluripotent stem cells: A novel tool to sustain pluripotency and facilitate differentiation.  Biomaterials  2021    PubMed ID: 33341735   DOI: 10.1016/j.biomaterials.2020.120575
4048  Shibata S, Hayashi R, Okubo T, Kudo Y, Katayama T, Ishikawa Y, Toga J, Yagi E, Honma Y, Quantock AJ, Sekiguchi K, Nishida K.  Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages  Cell Rep  2018  25(6):1668-1679  PubMed ID: 30404017   DOI: 10.1016/j.celrep.2018.10.032

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