Cell No. : Cell Name
HPS0522 : CiRA00169
update : 2023/06/07
|
| Comment | Disease specific iPS cell line derived from a patient : Achondroplasia. HPS0523 was derived from the same patient. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . 3) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 4) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
https://www.cira.kyoto-u.ac.jp/
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Age at sampling |
10s
|
| Tissue |
skin
|
| Disease name |
achondroplasia
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
pCE-hOct3/4, pCE-hSK, pCE-hUL, pCE-mp53DD, pCXB-EBNA1, Oct3/4, Sox2, Klf4, L-Myc, LIN28, p53DD, EBNA
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_UR06
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
Primate ES Cell Medium + 4ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin coated dish
|
Culture information
|
Passage ratio |
|
1 : 3-5 split
|
| SC frequency |
|
Subculture : once/4-6 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.5-2.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Relational File |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
Back
Top
CELL BANK Website
SDS_DAP(MEM)_Japanese.pdf