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Cell No. : Cell Name
HPS0508 : CiRA00161  update : 2023/06/07
CommentDisease specific iPS cell line derived from a patient : Charcot-Marie-Tooth disease. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . 3) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 4) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor https://www.cira.kyoto-u.ac.jp/
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 40s
Tissue blood mononuclear cell
Disease name charcot-Marie-Tooth disease
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, EBNA1, mp53DD
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_IP57
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma/Achoreplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 0
User's Publication 0


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