Cell No. : Cell Name
HPS0450 :
update : 2023/03/23
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| Comment | Disease specific iPS cell line derived from a patient : Juvenile nephronophthisis. The analysis performed by RIKEN BRC indicated homozygous deletion from 110,852,753 (MALL gene region) to 110,986,718 (NPHP1 gene region) in Chromosome 2 (detected by CGH array). HPS0449, HPS0450, HPS0451 were derived from the same patient. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . 3) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2013
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
30s
|
| Tissue |
peripheral blood mononuclear cell
|
| Disease name |
Juvenile nephronophthisis
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, mp53DD, EBNA1
|
| Vector |
pCE-hOCT3/4, pCE-hSK, pCE-hUL, pCE-mp53DD, pCXB-EBNA1
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_ZE69
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
| Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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Culture information
|
SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
STEM-CELLBANKER
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
1
|
| User's Publication |
|
9897
Yutaka Arai, Miho Takami, Yuri An, Mami Matsuo-Takasaki, Yasuko Hemmi, Tamami Wakabayashi, Jun Inoue, Michiya Noguchi, Yukio Nakamura, Keisuke Sugimoto, Tsukasa Takemura, Keisuke Okita, Kenji Osafune, Minoru Takasato, Tadayoshi Hayata, Yohei Hayashi
Generation of Two Human Induced Pluripotent Stem Cell Lines Derived From Two Juvenile Nephronophthisis Patients With NPHP1 Deletion
Stem Cell Res
2020
45:101815
PubMed ID: 32361464
DOI: 10.1016/j.scr.2020.101815
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