Cell No. : Cell Name
HPS0387 : CiRA00111-CKI-C2
update : 2024/04/12
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| Comment | Disease specific iPS cell line derived from a patient : Muscular dystrophy, Duchenne type. Correction of the dystrophin gene. HPS0383, HPS0384, HPS0385 and HPS0386 were derived from the same patient. Cryopreserved by vitrification method. |
| Comment from the depositor | To restore Dystrophin gene, exon 44 was inserted in front of the exon 45 via CRISPR-Cas9 mediated homologous recombination. Hygromycin resistance cassette was removed by Cre recombinase, hence a single loxP sequence remain in intron 44. |
| Terms and conditions | 1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies) . 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) Contact Us : RIKEN Cell Bank |
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0065.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
https://www.cira.kyoto-u.ac.jp/
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Male
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| Age at sampling |
1-9 years
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| Tissue |
skin
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| Disease name |
Muscular dystrophy, Duchenne type
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53 shRNA
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| Vector |
pCXLE-hOct3/4-SHP53-F, pCXLE-hSK, pCXLE-hUL
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Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_VM72
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (2.5mM GlutaMAX) + 2mM L-Glutamine + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 10ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
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13845
Minas Nalbandian, Mingming Zhao, Mitsuru Sasaki-Honda, Tatsuya Jonouchi, Antonio Lucena-Cacace, Takuma Mizusawa, Masahiko Yasuda, Yoshinori Yoshida, Akitsu Hotta, Hidetoshi Sakurai
Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy
Stem Cell Reports
2021
16(4):883-898
PubMed ID: 33798449
DOI: 10.1016/j.stemcr.2021.03.004
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4007
Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A.
Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9.
Stem Cell Reports
2015
4:143-54
PubMed ID: 25434822
DOI: 10.1016/j.stemcr.2014.10.013
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