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Cell No. : Cell Name
HPS0386 : CiRA00111-TKI-I15  update : 2023/06/07
CommentDisease specific iPS cell line derived from a patient : Muscular dystrophy, Duchenne type. Correction of the dystrophin gene. HPS0383, HPS0384, HPS0385 and HPS0387 were derived from the same patient. Cryopreserved by vitrification method.
Comment from the depositorTo restore Dystrophin gene, exon 44 was inserted in front of the exon 45 via TALEN mediated homologous recombination. Hygromycin resistance cassette was removed by Cre recombinase, hence a single loxP sequence remain in intron 44.
Terms and conditions1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies) . 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor https://www.cira.kyoto-u.ac.jp/
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 1-9 years
Tissue skin
Disease name Muscular dystrophy, Duchenne type
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53 shRNA
Vector pCXLE-hOct3/4-SHP53-F, pCXLE-hSK, pCXLE-hUL
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_VM75
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2.5mM GlutaMAX) + 2mM L-Glutamine + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 10ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 2
User's Publication 0


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Reference
13845  Minas Nalbandian, Mingming Zhao, Mitsuru Sasaki-Honda, Tatsuya Jonouchi, Antonio Lucena-Cacace, Takuma Mizusawa, Masahiko Yasuda, Yoshinori Yoshida, Akitsu Hotta, Hidetoshi Sakurai  Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy  Stem Cell Reports  2021  16(4):883-898  PubMed ID: 33798449   DOI: 10.1016/j.stemcr.2021.03.004
4007  Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A.  Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9.  Stem Cell Reports  2015  4:143-54   PubMed ID: 25434822   DOI: 10.1016/j.stemcr.2014.10.013

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