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細胞材料の再培養方法 ( HPS以外・ HPS )
細胞番号 : 細胞名
HPS0381 : 1231A3  update : 2024/11/11
細胞特性(Comment:英)Human iPS cell line derived from a healthy individual. HPS0379, HPS0380, HPS0381, HPS4293 was derived from the same person.
細胞特性(日)ヒト人工多能性幹(iPS)細胞株。HPS0379, HPS0380, HPS0381, HPS4293は同一人由来。Feeder free培養。緩慢冷却法保存。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
使用条件(日)1) 非営利機関以外の利用者は事前にiPSアカデミアジャパン株式会社 から確認書を得ること。2) 別紙1 (非営利) または別紙1 (営利) を提供同意書 (C-0003またはC-0003p) に添付すること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0041.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Yamanaka, Shinya
寄託日 2013
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 African/American
性別 Female
齢 29
採取組織 peripheral blood
細胞分類 iPS
遺伝子改変 recombinant
細胞寿命 infinite
細胞形態 ES-like
Cellosaurus(Expasy) CVCL_LJ39
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 StemFit AK02N
抗生物質 Free
継代方法 TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
培養容器のコーティング 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
播種細胞数 2.6-26x10 4 cells/60 mm dish
継代・培地交換頻度 Subculture : once/5-8 days, Medium Renewal : every day or every other day
培養最適温度 37 ℃
二酸化炭素濃度 5 %
フィーダー細胞 Free
凍結培地 StemFit AK02N + 10 μM Y-27632 + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ/アコレプラズマ (-)
ウィルス（HIV) Undetected
ウィルス（HTLV-1) (-)
染色体検査 46,XX,inv(9)(p12q13) 46,XX,inv(9)(p12q13) [20] at passage No. 14
その他 Use 10μM Y-27632 when passaging and thawing. Remove it next day.
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 20件
利用者成果(日) 0件

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Reference(英)
20937  Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.  In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.  PLoS One  2022  17(11)6:e0275600  PubMed ID: 36378656   DOI: 10.1371/journal.pone.0275600
4002  Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.  Sci Rep  2014  4:3594  PubMed ID: 24399248   DOI: 10.1038/srep03594

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Reference(日)

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利用者成果(英)
19706  Bamba K, Ozawa M, Daitoku H, Kohara A.  Diverting the food-freezing technology improves the cryopreservation efficiency of induced pluripotent stem cells and derived neurospheres  Regen Ther  2024  27:83-91  PubMed ID: 38525239   DOI: 10.1016/j.reth.2024.03.007
21586  Oonishi T, Nishimura K, Takata K, Fujimuro M, Sekine Y.  Scraping Assay as a Novel Strategy to Evaluate Axonal Regeneration Using Human-Induced Pluripotent Stem Cell-Derived Neurons.  Biol Pharm Bull  2024  47(2):366-372  PubMed ID: 38325853   DOI: 10.1248/bpb.b23-00675
21612  Gima S, Oe K, Nishimura K, Ohgita T, Ito H, Kimura H, Saito H, Takata K.  Host-to-graft propagation of inoculated α-synuclein into transplanted human induced pluripotent stem cell-derived midbrain dopaminergic neurons.  Regen Ther  2024  25:229-237  PubMed ID: 38283940   DOI: 10.1016/j.reth.2023.12.019
21915  Wang J, Morita K, Iwata T.  Induction of periodontal ligament-derived mesenchymal stromal cell-like cells from human induced pluripotent stem cells  Regen Ther  2024  26:432-441  PubMed ID: 39045575   DOI: 10.1016/j.reth.2024.05.005
22113  Li R, Tsuboi H, Ito H, Takagi D, Chang YH, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Ohnuma K, Takahashi S, Hayashi Y.  Generation of human induced pluripotent stem cell lines derived from two glucose transporter 1 deficiency syndrome patients.  Stem Cell Res  2024  81:103584  PubMed ID: 39490212   DOI: 10.1016/j.scr.2024.103584
22171  Matsuo-Takasaki M, Kambayashi S, Hemmi Y, Wakabayashi T, Shimizu T, An Y, Ito H, Takeuchi K, Ibuki M, Kawashima T, Masayasu R, Suzuki M, Kawai Y, Umekage M, Kato TM, Noguchi M, Nakade K, Nakamura Y, Nakaishi T, Nishishita N, Tsukahara M, Hayashi Y.  Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.  Elife  2024  12  PubMed ID: 39529479   DOI: 10.7554/eLife.89724
17772  Lin W, Shiomoto S, Yamada S, Watanabe H, Kawashima Y, Eguchi Y, Muramatsu K, Sekino Y.  Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons  iScience  2023  26(4):106285  PubMed ID: 37034988   DOI: 10.1016/j.isci.2023.106285
21050  Fukuda A, Nakashima S, Oda Y, Nishimura K, Kawashima H, Kimura H, Ohgita T, Kawashita E, Ishihara K, Hanaki A, Okazaki M, Matsuda E, Tanaka Y, Nakamura S, Matsumoto T, Akiba S, Saito H, Matsuda H, Takata K.  Plantainoside B in Bacopa monniera Binds to Aβ Aggregates Attenuating Neuronal Damage and Memory Deficits Induced by Aβ.  Biol Pharm Bull  2023  46(2):320-333  PubMed ID: 36724960   DOI: 10.1248/bpb.b22-00797
21085  Inoue S, Nishimura K, Gima S, Nakano M, Takata K.  CRISPR-Cas9-Edited SNCA Knockout Human Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons and Their Vulnerability to Neurotoxicity.  Biol Pharm Bull  2023  46(3):517-522  PubMed ID: 36858582   DOI: 10.1248/bpb.b22-00839
21123  Takata M, Nishimura K, Harada K, Iwasaki R, Ando M, Yamada S, Ginhoux F, Takata K.  Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems.  Tissue Cell  2023  81:102023  PubMed ID: 36709697   DOI: 10.1016/j.tice.2023.102023
21260  Taga S, Suga H, Nakano T, Kuwahara A, Inoshita N, Kodani Y, Nagasaki H, Sato Y, Tsumura Y, Sakakibara M, Soen M, Miwata T, Ozaki H, Kano M, Watari K, Ikeda A, Yamanaka M, Takahashi Y, Kitamoto S, Kawaguchi Y, Miyata T, Kobayashi T, Sugiyama M, Onoue T, Yasuda Y, Hagiwara D, Iwama S, Tomigahara Y, Kimura T, Arima H.  Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation.  Stem Cell Reports  2023    PubMed ID: 37295423   DOI: 10.1016/j.stemcr.2023.05.002
21294  Kato T, Nishimura K, Hirao M, Shimohama S, Takata K.  Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells.  Neuropsychopharmacol Rep  2023    PubMed ID: 37366076   DOI: 10.1002/npr2.12361
21311  Kaneko H, Kaitsuka T, Tomizawa K.  Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.  Cell Mol Life Sci  2023  80(8):200  PubMed ID: 37421441   DOI: 10.1007/s00018-023-04847-z
21322  Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.  Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.  Sci Rep  2023  13(1):11610  PubMed ID: 37463955   DOI: 10.1038/s41598-023-38548-1
21492  Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.  Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.  Cell Rep  2023  113431  PubMed ID: 38039961   DOI: 10.1016/j.celrep.2023.113431
21522  Wu Y, Iwasaki K, Hashimoto Y.  Induction of mesenchymal stem cell (MSC) differentiation from iPS cells using MSC medium.  Dent Mater J  2023    PubMed ID: 38008439   DOI: 10.4012/dmj.2023-130
20573  Nakano Y, Susa K, Yanagi T, Hiraoka Y, Suzuki T, Mori T, Ando F, Mandai S, Fujiki T, Rai T, Uchida S, Sohara E.  Generation of NPHP1 knockout human pluripotent stem cells by a practical biallelic gene deletion strategy using CRISPR/Cas9 and ssODN.  In Vitro Cell Dev Biol Anim  2022    PubMed ID: 35165826   DOI: 10.1007/s11626-022-00655-0
16698  Kakihana T, Takahashi M, Katsuragi Y, Yamashita SI, Sango J, Kanki T, Onodera O, Fujii M.  The optineurin/TIA1 pathway inhibits aberrant stress granule formation and reduces ubiquitinated TDP-43  iScience  2021  24(7):102733  PubMed ID: 34258561   DOI: 10.1016/j.isci.2021.102733
16864  Kenzo BAMBA, Midori OZAWA, Miho TAMAI, Emiko SHIMBO, Hiromasa SHINDO, Takuo OTA, Sungho AHN, Arihiro KOHARA, Yoh-ichi TAGAWA  Human serum albumin-containing xeno-free freezing medium optimized for regenerative medicine  Translational and Regulatory Sciences  2021  3:77-82    DOI: 10.33611/trs.2021-008
11055  Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K.  Automated Adherent Cell Elimination by a High-Speed Laser Mediated by a LightResponsive Polymer  Commun Biol  2018  1:218  PubMed ID: 30534610   DOI: 10.1038/s42003-018-0222-4

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