細胞番号 : 細胞名
HPS0381 : 1231A3
update : 2023/09/22
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| 細胞特性(Comment:英) | Human iPS cell line derived from healthy individual. HPS0379, HPS0380, HPS0381, HPS4293 was derived from the same person. |
| 細胞特性(日) | ヒト人工多能性幹(iPS)細胞株。HPS0379, HPS0380, HPS0381, HPS4293は同一人由来。Feeder free培養。緩慢冷却法保存。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . |
| 使用条件(日) | 1) 非営利機関以外の利用者は事前にiPSアカデミアジャパン株式会社 から確認書を得ること。2) 別紙1 (非営利) または別紙1 (営利) を提供同意書 (C-0003またはC-0003p) に添付すること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0041.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Yamanaka, Shinya
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| 寄託日 |
2013
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 人種 |
African/American
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| 性別 |
Female
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| 齢 |
29
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| 採取組織 |
peripheral blood
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| 細胞分類 |
iPS
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| 遺伝子改変 |
recombinant
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細胞寿命 |
infinite
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| 細胞形態 |
ES-like
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| Cellosaurus(Expasy) |
CVCL_LJ39
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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StemFit AK02N
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| 抗生物質 |
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Free
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継代方法 |
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TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
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| 培養容器のコーティング |
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0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
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播種細胞数 |
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2.6-26x10 4 cells/60 mm dish
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| 継代・培地交換頻度 |
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Subculture : once/5-8 days, Medium Renewal : every day or every other day
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| 培養最適温度 |
|
37
℃
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| 二酸化炭素濃度 |
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5
%
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| フィーダー細胞 |
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Free
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| 凍結培地 |
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StemFit AK02N + 10 μM Y-27632 + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| ウィルス（HIV) |
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Undetected
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| ウィルス（HTLV-1) |
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(-)
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| 染色体検査 |
46,XX,inv(9)(p12q13)
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46,XX,inv(9)(p12q13) [20] at passage No. 14
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| その他 |
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Use 10μM Y-27632 when passaging and thawing. Remove it next day.
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
2件
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| Reference(日) |
0件
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| 利用者成果(英) |
10件
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| 利用者成果(日) |
0件
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| Reference(英) |
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20937
Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.
In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.
PLoS One
2022
17(11)6:e0275600
PubMed ID: 36378656
DOI: 10.1371/journal.pone.0275600
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4002
Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.
Sci Rep
2014
4:3594
PubMed ID: 24399248
DOI: 10.1038/srep03594
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| 利用者成果(英) |
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17772
Lin W, Shiomoto S, Yamada S, Watanabe H, Kawashima Y, Eguchi Y, Muramatsu K, Sekino Y.
Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons
iScience
2023
26(4):106285
PubMed ID: 37034988
DOI: 10.1016/j.isci.2023.106285
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21050
Fukuda A, Nakashima S, Oda Y, Nishimura K, Kawashima H, Kimura H, Ohgita T, Kawashita E, Ishihara K, Hanaki A, Okazaki M, Matsuda E, Tanaka Y, Nakamura S, Matsumoto T, Akiba S, Saito H, Matsuda H, Takata K.
Plantainoside B in Bacopa monniera Binds to Aβ Aggregates Attenuating Neuronal Damage and Memory Deficits Induced by Aβ.
Biol Pharm Bull
2023
46(2):320-333
PubMed ID: 36724960
DOI: 10.1248/bpb.b22-00797
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21085
Inoue S, Nishimura K, Gima S, Nakano M, Takata K.
CRISPR-Cas9-Edited SNCA Knockout Human Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons and Their Vulnerability to Neurotoxicity.
Biol Pharm Bull
2023
46(3):517-522
PubMed ID: 36858582
DOI: 10.1248/bpb.b22-00839
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21123
Takata M, Nishimura K, Harada K, Iwasaki R, Ando M, Yamada S, Ginhoux F, Takata K.
Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems.
Tissue Cell
2023
81:102023
PubMed ID: 36709697
DOI: 10.1016/j.tice.2023.102023
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21260
Taga S, Suga H, Nakano T, Kuwahara A, Inoshita N, Kodani Y, Nagasaki H, Sato Y, Tsumura Y, Sakakibara M, Soen M, Miwata T, Ozaki H, Kano M, Watari K, Ikeda A, Yamanaka M, Takahashi Y, Kitamoto S, Kawaguchi Y, Miyata T, Kobayashi T, Sugiyama M, Onoue T, Yasuda Y, Hagiwara D, Iwama S, Tomigahara Y, Kimura T, Arima H.
Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation.
Stem Cell Reports
2023
PubMed ID: 37295423
DOI: 10.1016/j.stemcr.2023.05.002
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21294
Kato T, Nishimura K, Hirao M, Shimohama S, Takata K.
Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells.
Neuropsychopharmacol Rep
2023
PubMed ID: 37366076
DOI: 10.1002/npr2.12361
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21311
Kaneko H, Kaitsuka T, Tomizawa K.
Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.
Cell Mol Life Sci
2023
80(8):200
PubMed ID: 37421441
DOI: 10.1007/s00018-023-04847-z
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21322
Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.
Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.
Sci Rep
2023
13(1):11610
PubMed ID: 37463955
DOI: 10.1038/s41598-023-38548-1
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20573
Nakano Y, Susa K, Yanagi T, Hiraoka Y, Suzuki T, Mori T, Ando F, Mandai S, Fujiki T, Rai T, Uchida S, Sohara E.
Generation of NPHP1 knockout human pluripotent stem cells by a practical biallelic gene deletion strategy using CRISPR/Cas9 and ssODN.
In Vitro Cell Dev Biol Anim
2022
PubMed ID: 35165826
DOI: 10.1007/s11626-022-00655-0
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16864
Kenzo BAMBA, Midori OZAWA, Miho TAMAI, Emiko SHIMBO, Hiromasa SHINDO, Takuo OTA, Sungho AHN, Arihiro KOHARA, Yoh-ichi TAGAWA
Human serum albumin-containing xeno-free freezing medium optimized for regenerative medicine
Translational and Regulatory Sciences
2021
3:77-82
DOI: 10.33611/trs.2021-008
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